caa a22 4500 445376570 CHVBK 20180317143004.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00702-010-0560-y doi (NATIONALLICENCE)springer-10.1007/s00702-010-0560-y Identification of the disulfide bonds of lysyl oxidase [Elektronische Daten] [Xi Chen, Frederick Greenaway] Proteolytic digestion of bovine aortic lysyl oxidase followed by tandem mass spectrometry has enabled assignment of all five disulfide bonds. The results indicate that the enzyme has a very stable central core containing three disulfide bonds, the lysyl tyrosyl quinone cross-link and the copper. This core is well isolated from solvent with the result that the oxidized (normal) form of the enzyme is remarkably resistant to proteolysis and is unusually stable at high temperatures and in the presence of denaturants. Springer-Verlag, 2010 Lysyl oxidase nationallicence Disulfide linkages nationallicence Cysteine content nationallicence Tandem mass spectrometry nationallicence ABC : Ammonium bicarbonate nationallicence BNPS-Skatole : 2-(2-Nitrophenylsulfenyl)-3-methyl-3-bromoindolenine nationallicence LC/MS/MS : Tandem mass spectrometry nationallicence LOX : Lysyl oxidase nationallicence LOXL : Lysyl oxidase-like protein nationallicence LTQ : Lysyl tyrosyl quinone nationallicence TFA : Trifluoroacetic acid nationallicence TPQ : Topaquinone nationallicence Chen Xi Carlson School of Chemistry and Biochemistry, Clark University, 950 Main St, 01610-1477, Worcester, MA, USA aut Greenaway Frederick Carlson School of Chemistry and Biochemistry, Clark University, 950 Main St, 01610-1477, Worcester, MA, USA aut Journal of Neural Transmission Springer Vienna 118/7(2011-07-01), 1111-1114 0300-9564 118:7<1111 2011 118 702 https://doi.org/10.1007/s00702-010-0560-y text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00702-010-0560-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Xi Carlson School of Chemistry and Biochemistry, Clark University, 950 Main St, 01610-1477, Worcester, MA, USA aut NATIONALLICENCE 700 1- Greenaway Frederick Carlson School of Chemistry and Biochemistry, Clark University, 950 Main St, 01610-1477, Worcester, MA, USA aut NATIONALLICENCE 773 0- Journal of Neural Transmission Springer Vienna 118/7(2011-07-01), 1111-1114 0300-9564 118:7<1111 2011 118 702 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer