caa a22 4500 445376724 CHVBK 20180317143005.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00702-010-0505-5 doi (NATIONALLICENCE)springer-10.1007/s00702-010-0505-5 Active cortical innervation protects striatal neurons from slow degeneration in culture [Elektronische Daten] [Ianai Fishbein, Menahem Segal] Spiny striatal GABAergic neurons receive most of their excitatory input from the neocortex. In culture, striatal neurons form inhibitory connections, but the lack of intrinsic excitatory afferents prevents the development of spontaneous network activity. Addition of cortical neurons to the striatal culture provides the necessary excitatory input to the striatal neurons, and in the presence of these neurons, striatal cultures do express spontaneous network activity. We have confirmed that cortical neurons provide excitatory drive to striatal neurons in culture using paired recording from cortical and striatal neurons. In the presence of tetrodotoxin (TTX), which blocks action potential discharges, the connections between cortical and striatal neurons are still formed, and in fact synaptic currents generated between them when TTX is removed are far larger than in control, undrugged cultures. Interestingly, the continuous presence of TTX in the co-culture caused striatal cell death. These observations indicate that the mere presence of cortical neurons is not sufficient to preserve striatal neurons in culture, but their synchronous activity, triggered by cortical excitatory synapses, is critical for the maintenance of viability of striatal neurons. These results have important implications for understanding the role of activity in neurodegenerative diseases of the striatum. Springer-Verlag, 2010 Striatum nationallicence Culture nationallicence Cortex nationallicence Excitation nationallicence Survival nationallicence Fishbein Ianai Department of Neurobiology, The Weizmann Institute, 76100, Rehovot, Israel aut Segal Menahem Department of Neurobiology, The Weizmann Institute, 76100, Rehovot, Israel aut Journal of Neural Transmission Springer Vienna 118/3(2011-03-01), 445-451 0300-9564 118:3<445 2011 118 702 https://doi.org/10.1007/s00702-010-0505-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00702-010-0505-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fishbein Ianai Department of Neurobiology, The Weizmann Institute, 76100, Rehovot, Israel aut NATIONALLICENCE 700 1- Segal Menahem Department of Neurobiology, The Weizmann Institute, 76100, Rehovot, Israel aut NATIONALLICENCE 773 0- Journal of Neural Transmission Springer Vienna 118/3(2011-03-01), 445-451 0300-9564 118:3<445 2011 118 702 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer