caa a22 4500 445376805 CHVBK 20180317143005.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00702-010-0474-8 doi (NATIONALLICENCE)springer-10.1007/s00702-010-0474-8 Running wheel activity restores MPTP-induced functional deficits [Elektronische Daten] [Anders Fredriksson, Ingels Stigsdotter, Anders Hurtig, Béatrice Ewalds-Kvist, Trevor Archer] Wheel-running and treadmill running physical exercise have been shown to alleviate parkinsonism in both laboratory and clinical studies. MPTP was administered to C57/BL6 mice using two different procedures: (a) administration of a double-dose regime (MPTP 2×20 or 2×40mg/kg, separated by a 24-h interval), vehicle (saline 5ml/kg) or saline (vehicle 2×5ml/kg), and (b) administration of a single-dose weekly regime (MPTP 1×40mg/kg) or saline (vehicle 1×5ml/kg) repeated over 4 consecutive weeks. For each procedure, two different physical exercise regimes were followed: (a) after the double-dose MPTP regime, mice were given daily 30-min periods of wheel-running exercise over 5 consecutive days/week or placed in a cage in close proximity to the running wheels for 3weeks. (b) Mice were either given wheel-running activity on 4 consecutive days (30-min periods) or placed in a cage nearby for 14weeks. Behavioral testing was as follows: (a) after 3weeks of exercise/no exercise, mice were tested for spontaneous motor activity (60min) and subthreshold l-Dopa (5mg/kg)-induced activity. (b) Spontaneous motor activity was measured on the fifth day during each of the each of the first 5weeks (Tests 1-5), about 1h before injections (first 4weeks), and continued on the 5th days of the 6th to the 14th weeks (Tests 6-14). Subthreshold l-Dopa (5mg/kg)-induced activity was tested on the 6th, 8th, 10th, 12th and 14th weeks. (b) Mice from the single-dose MPTP weekly regime were killed during the 15th week and striatal regions taken for dopamine analysis, whereas frontal and parietal cortex and hippocampus were taken for analysis of brain-derived neurotrophic factor (BDNF). It was shown that in both experiments, i.e., the double-dose regime and single-dose weekly regime of MPTP administration, physical activity attenuated markedly the MPTP-induced akinesia/hypokinesia in both the spontaneous motor activity and restored motor activity completely in subthreshold l-Dopa tests. Running wheel activity attenuated markedly the loss of dopamine due to repeated administrations of MPTP. BDNF protein level in the parietal cortex was elevated by the MPTP insult and increased further by physical exercise. Physical running wheel exercise alleviated both the functional and biomarker expressions of MPTP-induced parkinsonism. Springer-Verlag, 2010 Exercise nationallicence Running wheel nationallicence MPTP nationallicence Motor activity nationallicence l -Dopa nationallicence Locomotion nationallicence Rearing nationallicence Motor activity nationallicence Restoration nationallicence Dopamine nationallicence BDNF nationallicence C57/BL6 mice nationallicence Fredriksson Anders Department of Neuroscience Psychiatry, Uppsala University, 751 85, Uppsala, Sweden aut Stigsdotter Ingels School of Health and Social Studies, University of Dalarna, 791 88, Falun, Sweden aut Hurtig Anders School of Health and Social Studies, University of Dalarna, 791 88, Falun, Sweden aut Ewalds-Kvist Béatrice Department of Psychology, University of Stockholm, 106 91, Stockholm, Sweden aut Archer Trevor Department of Psychology, University of Gothenburg, Box 500, 430 50, Gothenburg, Sweden aut Journal of Neural Transmission Springer Vienna 118/3(2011-03-01), 407-420 0300-9564 118:3<407 2011 118 702 https://doi.org/10.1007/s00702-010-0474-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00702-010-0474-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fredriksson Anders Department of Neuroscience Psychiatry, Uppsala University, 751 85, Uppsala, Sweden aut NATIONALLICENCE 700 1- Stigsdotter Ingels School of Health and Social Studies, University of Dalarna, 791 88, Falun, Sweden aut NATIONALLICENCE 700 1- Hurtig Anders School of Health and Social Studies, University of Dalarna, 791 88, Falun, Sweden aut NATIONALLICENCE 700 1- Ewalds-Kvist Béatrice Department of Psychology, University of Stockholm, 106 91, Stockholm, Sweden aut NATIONALLICENCE 700 1- Archer Trevor Department of Psychology, University of Gothenburg, Box 500, 430 50, Gothenburg, Sweden aut NATIONALLICENCE 773 0- Journal of Neural Transmission Springer Vienna 118/3(2011-03-01), 407-420 0300-9564 118:3<407 2011 118 702 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer