caa a22 4500 445377186 CHVBK 20180317143006.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00702-010-0520-6 doi (NATIONALLICENCE)springer-10.1007/s00702-010-0520-6 Down regulation of trophic factors in neonatal rat spinal cord after administration of cerebrospinal fluid from sporadic amyotrophic lateral sclerosis patients [Elektronische Daten] [P. Deepa, Neelam Shahani, Phalguni Alladi, K. Vijayalakshmi, T. Sathyaprabha, A. Nalini, V. Ravi, T. Raju] Accumulating evidence supports neuroprotective role of trophic factors in amyotrophic lateral sclerosis (ALS). Previous studies from our laboratory report that the CSF of patients with sporadic ALS (ALS-CSF) induces degenerative changes in the rat spinal motor neurons and reactive astrogliosis in the surrounding gray matter. The present study was aimed to investigate if the ALS-CSF affected the expression of trophic factors namely, brain-derived neurotrophic factor (BDNF), fibroblast growth factor 2 (FGF2) and insulin-like growth factor 1 (IGF1) in the newborn rat spinal cords. ALS-CSF was intrathecally injected into the neonatal rats and the mRNA levels of the trophic factors were determined by quantitative real-time polymerase chain reaction. Here, we report significant down regulation in the gene expression of trophic factors for BDNF, FGF2 and IGF1. BDNF mRNA levels were found to be reduced by 6.8-fold in the ALS-CSF injected group compared to control groups. The levels of IGF1 and FGF2 mRNA were also decreased by 3.91- and 2.13-fold, respectively, in the ALS group. We further found that exogenous supplementation of BDNF considerably reduced the aberrant phosphorylation of neurofilaments, complementing our earlier findings of restored expression of voltage gated sodium channel. Reduced expression of trophic factors indicates an altered microenvironment of the motor neurons and could possibly be one of the contributing factors in the degeneration process. Springer-Verlag, 2010 Sporadic ALS nationallicence BDNF nationallicence IGF1 nationallicence FGF2 nationallicence ALS-CSF nationallicence Rat spinal cord nationallicence Deepa P. Department of Neurophysiology, National Institute of Mental Health and Neurosciences, Hosur Road, PB # 2900, 560 029, Bangalore, India aut Shahani Neelam Department of Neurophysiology, National Institute of Mental Health and Neurosciences, Hosur Road, PB # 2900, 560 029, Bangalore, India aut Alladi Phalguni Department of Neurophysiology, National Institute of Mental Health and Neurosciences, Hosur Road, PB # 2900, 560 029, Bangalore, India aut Vijayalakshmi K. Department of Neurophysiology, National Institute of Mental Health and Neurosciences, Hosur Road, PB # 2900, 560 029, Bangalore, India aut Sathyaprabha T. Department of Neurophysiology, National Institute of Mental Health and Neurosciences, Hosur Road, PB # 2900, 560 029, Bangalore, India aut Nalini A. Department of Neurology, National Institute of Mental Health and Neurosciences, Hosur Road, PB # 2900, 560 029, Bangalore, India aut Ravi V. Department of Neurovirology, National Institute of Mental Health and Neurosciences, Hosur Road, PB # 2900, 560 029, Bangalore, India aut Raju T. Department of Neurophysiology, National Institute of Mental Health and Neurosciences, Hosur Road, PB # 2900, 560 029, Bangalore, India aut Journal of Neural Transmission Springer Vienna 118/4(2011-04-01), 531-538 0300-9564 118:4<531 2011 118 702 https://doi.org/10.1007/s00702-010-0520-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00702-010-0520-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Deepa P. Department of Neurophysiology, National Institute of Mental Health and Neurosciences, Hosur Road, PB # 2900, 560 029, Bangalore, India aut NATIONALLICENCE 700 1- Shahani Neelam Department of Neurophysiology, National Institute of Mental Health and Neurosciences, Hosur Road, PB # 2900, 560 029, Bangalore, India aut NATIONALLICENCE 700 1- Alladi Phalguni Department of Neurophysiology, National Institute of Mental Health and Neurosciences, Hosur Road, PB # 2900, 560 029, Bangalore, India aut NATIONALLICENCE 700 1- Vijayalakshmi K. Department of Neurophysiology, National Institute of Mental Health and Neurosciences, Hosur Road, PB # 2900, 560 029, Bangalore, India aut NATIONALLICENCE 700 1- Sathyaprabha T. Department of Neurophysiology, National Institute of Mental Health and Neurosciences, Hosur Road, PB # 2900, 560 029, Bangalore, India aut NATIONALLICENCE 700 1- Nalini A. Department of Neurology, National Institute of Mental Health and Neurosciences, Hosur Road, PB # 2900, 560 029, Bangalore, India aut NATIONALLICENCE 700 1- Ravi V. Department of Neurovirology, National Institute of Mental Health and Neurosciences, Hosur Road, PB # 2900, 560 029, Bangalore, India aut NATIONALLICENCE 700 1- Raju T. Department of Neurophysiology, National Institute of Mental Health and Neurosciences, Hosur Road, PB # 2900, 560 029, Bangalore, India aut NATIONALLICENCE 773 0- Journal of Neural Transmission Springer Vienna 118/4(2011-04-01), 531-538 0300-9564 118:4<531 2011 118 702 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer