caa a22 4500 445377364 CHVBK 20180317143007.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00702-011-0596-7 doi (NATIONALLICENCE)springer-10.1007/s00702-011-0596-7 α-Synuclein increases U251 cells vulnerability to hydrogen peroxide by disrupting calcium homeostasis [Elektronische Daten] [Zhengxin Ying, Fankai Lin, Weihong Gu, Yang Su, Abida Arshad, Hong Qing, Yulin Deng] Multiple system atrophy (MSA) is a progressive neurodegenerative disease characterized by glial cytoplasmic inclusions containing insoluble α-synuclein. Since Ca2+ plays an important role in cell degeneration, [Ca2+]i in α-synuclein-overexpressed human glioma cells was analyzed by Fura-2 fluorometry. Overexpression of α-synuclein increased the basal level of [Ca2+]i, and a higher Ca2+ response to hydrogen peroxide was further observed. The effect that α-synuclein overexpression caused U251 cells to be more vulnerable to hydrogen peroxide was eliminated by Ca2+ chelator BAPTA-AM or transient receptor potential channels blocker SKF 96365 but not by L-type Ca2+ channel blocker nimodipine. These findings suggest that the dysregulation of cellular Ca2+ homeostasis caused by α-synuclein under oxidative stress may contribute to the glial cell death in MSA. Springer-Verlag, 2011 α-Synuclein nationallicence Calcium nationallicence Calcium channels nationallicence Multiple system atrophy nationallicence Ying Zhengxin School of Life Science, Beijing Institute of Technology, No. 5 Zhongguancun Nandajie, Haidian District, 100081, Beijing, People's Republic of China aut Lin Fankai School of Life Science, Beijing Institute of Technology, No. 5 Zhongguancun Nandajie, Haidian District, 100081, Beijing, People's Republic of China aut Gu Weihong Department of Neurology, Movement Disorder and Neurogenetics Research Center, China-Japan Friendship Hospital, Beijing, China aut Su Yang School of Life Science, Beijing Institute of Technology, No. 5 Zhongguancun Nandajie, Haidian District, 100081, Beijing, People's Republic of China aut Arshad Abida School of Life Science, Beijing Institute of Technology, No. 5 Zhongguancun Nandajie, Haidian District, 100081, Beijing, People's Republic of China aut Qing Hong School of Life Science, Beijing Institute of Technology, No. 5 Zhongguancun Nandajie, Haidian District, 100081, Beijing, People's Republic of China aut Deng Yulin School of Life Science, Beijing Institute of Technology, No. 5 Zhongguancun Nandajie, Haidian District, 100081, Beijing, People's Republic of China aut Journal of Neural Transmission Springer Vienna 118/8(2011-08-01), 1165-1172 0300-9564 118:8<1165 2011 118 702 https://doi.org/10.1007/s00702-011-0596-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00702-011-0596-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ying Zhengxin School of Life Science, Beijing Institute of Technology, No. 5 Zhongguancun Nandajie, Haidian District, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Lin Fankai School of Life Science, Beijing Institute of Technology, No. 5 Zhongguancun Nandajie, Haidian District, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Gu Weihong Department of Neurology, Movement Disorder and Neurogenetics Research Center, China-Japan Friendship Hospital, Beijing, China aut NATIONALLICENCE 700 1- Su Yang School of Life Science, Beijing Institute of Technology, No. 5 Zhongguancun Nandajie, Haidian District, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Arshad Abida School of Life Science, Beijing Institute of Technology, No. 5 Zhongguancun Nandajie, Haidian District, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Qing Hong School of Life Science, Beijing Institute of Technology, No. 5 Zhongguancun Nandajie, Haidian District, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Deng Yulin School of Life Science, Beijing Institute of Technology, No. 5 Zhongguancun Nandajie, Haidian District, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Neural Transmission Springer Vienna 118/8(2011-08-01), 1165-1172 0300-9564 118:8<1165 2011 118 702 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer