caa a22 4500 445377399 CHVBK 20180317143007.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00702-010-0543-z doi (NATIONALLICENCE)springer-10.1007/s00702-010-0543-z Different CSF β-amyloid processing in Alzheimer's and Creutzfeldt-Jakob disease [Elektronische Daten] [Brit Mollenhauer, Hermann Esselmann, Sigrun Roeber, Walter Schulz-Schaeffer, Claudia Trenkwalder, Mirko Bibl, Petra Steinacker, Hans Kretzschmar, Jens Wiltfang, Markus Otto] Decreased levels of β-amyloid (Aβ) 1-42 in cerebrospinal fluid (CSF) are characteristic for Alzheimer's disease (AD) and are also evident in Creutzfeldt-Jakob disease (CJD). Aβ plaques are thought to be responsible for this decrease in AD patients, whereas such Aβ plaques are rarely seen in CJD. To investigate the Aβ pattern in brain and CSF of neuropathologically confirmed CJD and AD patients we used an electrophoretic method to investigate Aβ peptide fractions which are not accessible to ELISA and immunohistochemistry. We analyzed Aβ peptides in the CSF of autopsy-confirmed CJD and AD patients and the corresponding brain homogenates using a quantitative urea-based Aβ electrophoresis immunoblot (Aβ-SDS-PAGE/immunoblot).The CSF Aβ1-42 decrease correlated with the brain Aβ load in AD, but not in CJD. There was no difference in the soluble fractions of brain homogenate in AD and CJD. We therefore conclude that different mechanisms in AD and CJD are responsible for the Aβ1-42 decrease in the CSF. Springer-Verlag, 2011 β-amyloid nationallicence β-amyloid plaques Creutzfeldt-Jakob disease nationallicence Alzheimer's disease nationallicence Cerebrospinal fluid nationallicence Mollenhauer Brit Paracelsus-Elena Klinik, Kassel and Georg-August University, Göttingen, Germany aut Esselmann Hermann Department of Psychiatry, Rheinische Kliniken Essen, University of Duisburg-Essen, Essen, Germany aut Roeber Sigrun Department of Neuropathology, Ludwig Maximilians University, Munich, Germany aut Schulz-Schaeffer Walter Department of Neuropathology, University Medical Center, Göttingen, Germany aut Trenkwalder Claudia Paracelsus-Elena Klinik, Kassel and Georg-August University, Göttingen, Germany aut Bibl Mirko Kliniken Essen Mitte, Department of Psychiatry, Psychotherapy and addiction medicine, University of Duisburg-Essen, Henricistrasse 92, 45136, Essen, Germany aut Steinacker Petra Department of Neurology, University of Ulm, Ulm, Germany aut Kretzschmar Hans Department of Neuropathology, Ludwig Maximilians University, Munich, Germany aut Wiltfang Jens Department of Psychiatry, Rheinische Kliniken Essen, University of Duisburg-Essen, Essen, Germany aut Otto Markus Department of Neurology, University of Ulm, Ulm, Germany aut Journal of Neural Transmission Springer Vienna 118/5(2011-05-01), 691-697 0300-9564 118:5<691 2011 118 702 https://doi.org/10.1007/s00702-010-0543-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00702-010-0543-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mollenhauer Brit Paracelsus-Elena Klinik, Kassel and Georg-August University, Göttingen, Germany aut NATIONALLICENCE 700 1- Esselmann Hermann Department of Psychiatry, Rheinische Kliniken Essen, University of Duisburg-Essen, Essen, Germany aut NATIONALLICENCE 700 1- Roeber Sigrun Department of Neuropathology, Ludwig Maximilians University, Munich, Germany aut NATIONALLICENCE 700 1- Schulz-Schaeffer Walter Department of Neuropathology, University Medical Center, Göttingen, Germany aut NATIONALLICENCE 700 1- Trenkwalder Claudia Paracelsus-Elena Klinik, Kassel and Georg-August University, Göttingen, Germany aut NATIONALLICENCE 700 1- Bibl Mirko Kliniken Essen Mitte, Department of Psychiatry, Psychotherapy and addiction medicine, University of Duisburg-Essen, Henricistrasse 92, 45136, Essen, Germany aut NATIONALLICENCE 700 1- Steinacker Petra Department of Neurology, University of Ulm, Ulm, Germany aut NATIONALLICENCE 700 1- Kretzschmar Hans Department of Neuropathology, Ludwig Maximilians University, Munich, Germany aut NATIONALLICENCE 700 1- Wiltfang Jens Department of Psychiatry, Rheinische Kliniken Essen, University of Duisburg-Essen, Essen, Germany aut NATIONALLICENCE 700 1- Otto Markus Department of Neurology, University of Ulm, Ulm, Germany aut NATIONALLICENCE 773 0- Journal of Neural Transmission Springer Vienna 118/5(2011-05-01), 691-697 0300-9564 118:5<691 2011 118 702 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer