caa a22 4500 445377623 CHVBK 20180317143007.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00702-010-0495-3 doi (NATIONALLICENCE)springer-10.1007/s00702-010-0495-3 Exercise increases neural stem cell proliferation surrounding the area of damage following rat traumatic brain injury [Elektronische Daten] [Tatsuki Itoh, Motohiro Imano, Shozo Nishida, Masahiro Tsubaki, Shigeo Hashimoto, Akihiko Ito, Takao Satou] Exercise enhances neuronal stem cell (NSC) proliferation and neurogenesis. However, the effect of exercise on NSC proliferation surrounding the area of damage after traumatic brain injury (TBI) is unknown. Here, we investigate the effect of running on NSC proliferation following TBI in the rat. Wistar rats received TBI and were randomly divided into two groups: (1) non-exercise group and (2) exercise group. The exercise group ran on a treadmill for 30min/day at 22m/min for 7 consecutive days. Immunohistochemistry was used to monitor NSC proliferation around the damaged area, and ex vivo techniques were used to isolate NSCs from the damaged region in both groups. The number of nestin- and Ki67-positive cells observed at 3 and 7days after TBI was significantly greater in the exercise group than in the non-exercise group (P<0.01). Furthermore, most nestin-positive cells in the exercise group co-localized with Ki67-positive cells. In ex vivo studies, spheres could be isolated from injured brain tissue from the exercise group at 3 and 7days following TBI, but at only 3days in the non-exercise group. The number of spheres isolated from injured brain tissue was greater in the exercise group than in the non-exercise group. Spheres were immunopositive for nestin and comprised NSCs that could differentiate into neurons and glia. Exercise increases the proliferation of NSCs around the damaged area following TBI. Therefore, exercise therapy (rehabilitation) in the early phase following TBI is important for recuperation from cerebral dysfunction induced by TBI. Springer-Verlag, 2010 Exercise nationallicence Neural stem cell nationallicence Traumatic brain injury nationallicence Nestin nationallicence Neurosphere nationallicence Itoh Tatsuki Department of Pathology, Kinki University School of Medicine, 377-2, Ohno-higashi, 589-8511, Osaka-Sayama, Osaka, Japan aut Imano Motohiro Department of Surgery, Kinki University School of Medicine, Osaka-Sayama, Osaka, Japan aut Nishida Shozo Kinki University School of Pharmaceutical Sciences, Osaka, Japan aut Tsubaki Masahiro Kinki University School of Pharmaceutical Sciences, Osaka, Japan aut Hashimoto Shigeo Division of Pathology, PL Hospital, Osaka, Japan aut Ito Akihiko Department of Pathology, Kinki University School of Medicine, 377-2, Ohno-higashi, 589-8511, Osaka-Sayama, Osaka, Japan aut Satou Takao Department of Pathology, Kinki University School of Medicine, 377-2, Ohno-higashi, 589-8511, Osaka-Sayama, Osaka, Japan aut Journal of Neural Transmission Springer Vienna 118/2(2011-02-01), 193-202 0300-9564 118:2<193 2011 118 702 https://doi.org/10.1007/s00702-010-0495-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00702-010-0495-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Itoh Tatsuki Department of Pathology, Kinki University School of Medicine, 377-2, Ohno-higashi, 589-8511, Osaka-Sayama, Osaka, Japan aut NATIONALLICENCE 700 1- Imano Motohiro Department of Surgery, Kinki University School of Medicine, Osaka-Sayama, Osaka, Japan aut NATIONALLICENCE 700 1- Nishida Shozo Kinki University School of Pharmaceutical Sciences, Osaka, Japan aut NATIONALLICENCE 700 1- Tsubaki Masahiro Kinki University School of Pharmaceutical Sciences, Osaka, Japan aut NATIONALLICENCE 700 1- Hashimoto Shigeo Division of Pathology, PL Hospital, Osaka, Japan aut NATIONALLICENCE 700 1- Ito Akihiko Department of Pathology, Kinki University School of Medicine, 377-2, Ohno-higashi, 589-8511, Osaka-Sayama, Osaka, Japan aut NATIONALLICENCE 700 1- Satou Takao Department of Pathology, Kinki University School of Medicine, 377-2, Ohno-higashi, 589-8511, Osaka-Sayama, Osaka, Japan aut NATIONALLICENCE 773 0- Journal of Neural Transmission Springer Vienna 118/2(2011-02-01), 193-202 0300-9564 118:2<193 2011 118 702 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer