caa a22 4500 445377828 CHVBK 20180317143008.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00702-011-0630-9 doi (NATIONALLICENCE)springer-10.1007/s00702-011-0630-9 A complex interaction between glycine/NMDA receptors and serotonergic/noradrenergic antidepressants in the forced swim test in mice [Elektronische Daten] [Ewa Poleszak, Piotr Wlaź, Bernadeta Szewczyk, Aleksandra Wlaź, Regina Kasperek, Andrzej Wróbel, Gabriel Nowak] Both clinical and preclinical studies demonstrate the antidepressant activity of the functional NMDA receptor antagonists. In this study, we assessed the effects of two glycine/NMDA receptor ligands, namely L-701,324 (antagonist) and d-cycloserine (a partial agonist) on the action of antidepressant drugs with different pharmacological profiles in the forced swim test in mice. Swim sessions were conducted by placing mice individually in glass cylinders filled with warmed water for 6min. The duration of behavioral immobility during the last 4min of the test was evaluated. The locomotor activity of mice was measured with photoresistor actimeters. L-701,324 and d-cycloserine given with reboxetine (administered in subeffective doses) did not change the behavior of animals in the forced swim test. A potentiating effect was seen when both tested glycine site ligands were given concomitantly with imipramine or fluoxetine in this test. The lesion of noradrenaline nerve terminals produced by DSP-4 neither altered the baseline activity nor influenced the antidepressant-like action of L-701,324 or d-cycloserine. The depletion of serotonin by p-CPA did not alter baseline activity in the forced swim test. However, it completely antagonized the antidepressant-like action produced by L-701,324 and d-cycloserine. Moreover, the antidepressant-like effects of imipramine, fluoxetine and reboxetine were abolished by d-serine, a full agonist of glycine/NMDA receptors. The present study demonstrates that glycine/NMDA receptor functional antagonists enhance the antidepressant-like action of serotonin, but not noradrenaline-based antidepressants and such their activity seems to depend on serotonin rather than noradrenaline pathway. The Author(s), 2011 NMDA receptor nationallicence Glycine site nationallicence Lesion nationallicence Antidepressants nationallicence Forced swim test nationallicence Mice nationallicence Poleszak Ewa Chair and Department of Applied Pharmacy, Medical University of Lublin, Chodźki 1, 20-093, Lublin, Poland aut Wlaź Piotr Department of Animal Physiology, Institute of Biology, Maria Curie-Skłodowska University, Akademicka 19, 20-033, Lublin, Poland aut Szewczyk Bernadeta Department of Neurobiology, Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland aut Wlaź Aleksandra Chair and Department of Applied Pharmacy, Medical University of Lublin, Chodźki 1, 20-093, Lublin, Poland aut Kasperek Regina Chair and Department of Applied Pharmacy, Medical University of Lublin, Chodźki 1, 20-093, Lublin, Poland aut Wróbel Andrzej Second Department of Gynecology, Medical University of Lublin, Lublin, Poland aut Nowak Gabriel Department of Neurobiology, Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland aut Journal of Neural Transmission Springer Vienna 118/11(2011-11-01), 1535-1546 0300-9564 118:11<1535 2011 118 702 https://doi.org/10.1007/s00702-011-0630-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00702-011-0630-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Poleszak Ewa Chair and Department of Applied Pharmacy, Medical University of Lublin, Chodźki 1, 20-093, Lublin, Poland aut NATIONALLICENCE 700 1- Wlaź Piotr Department of Animal Physiology, Institute of Biology, Maria Curie-Skłodowska University, Akademicka 19, 20-033, Lublin, Poland aut NATIONALLICENCE 700 1- Szewczyk Bernadeta Department of Neurobiology, Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland aut NATIONALLICENCE 700 1- Wlaź Aleksandra Chair and Department of Applied Pharmacy, Medical University of Lublin, Chodźki 1, 20-093, Lublin, Poland aut NATIONALLICENCE 700 1- Kasperek Regina Chair and Department of Applied Pharmacy, Medical University of Lublin, Chodźki 1, 20-093, Lublin, Poland aut NATIONALLICENCE 700 1- Wróbel Andrzej Second Department of Gynecology, Medical University of Lublin, Lublin, Poland aut NATIONALLICENCE 700 1- Nowak Gabriel Department of Neurobiology, Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland aut NATIONALLICENCE 773 0- Journal of Neural Transmission Springer Vienna 118/11(2011-11-01), 1535-1546 0300-9564 118:11<1535 2011 118 702 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer