caa a22 4500 445377836 CHVBK 20180317143008.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00702-011-0710-x doi (NATIONALLICENCE)springer-10.1007/s00702-011-0710-x Microarray expression profiling in 6-hydroxydopamine-induced dopaminergic neuronal cell death [Elektronische Daten] [Bokyung Park, Chang-Ki Oh, Won-Seok Choi, In Chung, Moussa Youdim, Young Oh] Parkinson's disease (PD) is the second most common neurodegenerative disorder and is characterized by a loss of dopaminergic neurons in the substantia nigra pars compacta. To discover potential key molecules in this process, we utilized cDNA microarray technology to obtain an expression profile of transcripts in MN9D dopaminergic neuronal cells treated with 6-hydroxydopamine. Using a self-organizing map algorithm, data mining and clustering were combined to identify distinct functional subgroups of genes. We identified alterations in the expression of 81 genes in eight clusters. Among these genes, we verified protein expression patterns of MAP kinase phosphatase 1 and sequestosome 1 using both cell culture and rat brain models of PD. Immunological analyses revealed increased expression levels as well as aggregated distribution patterns of these gene products in 6-hydroxydopamine-treated dopaminergic neurons. In addition to the identification of other proteins that are known to be associated with protein aggregation, our results raise the possibility that a more widespread set of proteins may be associated with the generation of protein aggregates in dying neurons. Further research to determine the functional roles of other altered gene products within the same cluster as well as the seven remaining clusters may provide new insights into the neurodegeneration that underlies PD pathogenesis. Springer-Verlag, 2011 Microarray nationallicence Dopaminergic neurons nationallicence 6-hydroxydopamine nationallicence MKP1 nationallicence Sequestosome 1 nationallicence Park Bokyung Department of Biology, Yonsei University College of Life Science and Biotechnology, 134 Shinchon-Dong, Seodaemoon-Gu, 120-749, Seoul, Korea aut Oh Chang-Ki Department of Biology, Yonsei University College of Life Science and Biotechnology, 134 Shinchon-Dong, Seodaemoon-Gu, 120-749, Seoul, Korea aut Choi Won-Seok Department of Biology, Yonsei University College of Life Science and Biotechnology, 134 Shinchon-Dong, Seodaemoon-Gu, 120-749, Seoul, Korea aut Chung In Department of Biology, Yonsei University College of Life Science and Biotechnology, 134 Shinchon-Dong, Seodaemoon-Gu, 120-749, Seoul, Korea aut Youdim Moussa Department of Biology, Yonsei University College of Life Science and Biotechnology, 134 Shinchon-Dong, Seodaemoon-Gu, 120-749, Seoul, Korea aut Oh Young Department of Biology, Yonsei University College of Life Science and Biotechnology, 134 Shinchon-Dong, Seodaemoon-Gu, 120-749, Seoul, Korea aut Journal of Neural Transmission Springer Vienna 118/11(2011-11-01), 1585-1598 0300-9564 118:11<1585 2011 118 702 https://doi.org/10.1007/s00702-011-0710-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00702-011-0710-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Park Bokyung Department of Biology, Yonsei University College of Life Science and Biotechnology, 134 Shinchon-Dong, Seodaemoon-Gu, 120-749, Seoul, Korea aut NATIONALLICENCE 700 1- Oh Chang-Ki Department of Biology, Yonsei University College of Life Science and Biotechnology, 134 Shinchon-Dong, Seodaemoon-Gu, 120-749, Seoul, Korea aut NATIONALLICENCE 700 1- Choi Won-Seok Department of Biology, Yonsei University College of Life Science and Biotechnology, 134 Shinchon-Dong, Seodaemoon-Gu, 120-749, Seoul, Korea aut NATIONALLICENCE 700 1- Chung In Department of Biology, Yonsei University College of Life Science and Biotechnology, 134 Shinchon-Dong, Seodaemoon-Gu, 120-749, Seoul, Korea aut NATIONALLICENCE 700 1- Youdim Moussa Department of Biology, Yonsei University College of Life Science and Biotechnology, 134 Shinchon-Dong, Seodaemoon-Gu, 120-749, Seoul, Korea aut NATIONALLICENCE 700 1- Oh Young Department of Biology, Yonsei University College of Life Science and Biotechnology, 134 Shinchon-Dong, Seodaemoon-Gu, 120-749, Seoul, Korea aut NATIONALLICENCE 773 0- Journal of Neural Transmission Springer Vienna 118/11(2011-11-01), 1585-1598 0300-9564 118:11<1585 2011 118 702 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer