caa a22 4500 445377852 CHVBK 20180317143008.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00702-011-0645-2 doi (NATIONALLICENCE)springer-10.1007/s00702-011-0645-2 Repeated co-administrations of alcohol- and methamphetamine-produced anxiogenic effect could be associated with the neurotoxicity in the dentate gyrus [Elektronische Daten] [Jia-Ying Chuang, Wan-Ting Chang, Chianfang Cherng, Gour-Shenq Kao, Lung Yu] To date, joint use of alcohol (EtOH) and methamphetamine (MA) represents a specific combination of polydrug abuse. Repeated administrations of EtOH, MA, and combined EtOH and MA were assessed for their effects on brain cell toxicity, cell mitosis and anxiety/depression-like behavior. We found that repeated co-administrations of EtOH and MA produced profound anxiogenic effects. Specifically, combined EtOH and MA decreased open arm exploratory responses in the elevated plus maze test. Moreover, combined EtOH and MA significantly decreased immobile responses in the tail suspension test. MA, EtOH, and their combination all reduced the number of NeuN-positive cells in amygdala (Amg), while neither of them altered the number of NeuN-positive cells in striatum (St) or prefrontal cortex (PFC). Combined EtOH and MA decreased the number of NeuN-positive cells in dentate gyrus (DG). EtOH, MA, and combined EtOH and MA all diminished comparable number of GFAP-positive cells in Amg, DG, and St. Neither of these treatment affected the number of GFAP-positive cells in PFC. EtOH, MA, and combined EtOH and MA generated comparable inhibiting effects on cell proliferation in the subventricular zone (SVZ) and DG. These results, taken together, suggest that repeated co-administrations of MA and EtOH may produce an observable anxiogenic effect. This combination-produced anxiogenic effect could be associated with neuronal loss in the dentate gurus. In contrast, such an anxiogenic effect is less likely related to this combination-caused glial toxicity in limbic regions or cell proliferation-inhibiting effect in the SVZ or DG. Springer-Verlag, 2011 Neurotoxicity nationallicence Methamphetamine nationallicence Ethanol nationallicence Dopamine nationallicence Polydrug nationallicence Anxiety nationallicence Chuang Jia-Ying Institute of Basic Medical Sciences, National Cheng Kung University College of Medicine, 70101, Tainan, Taiwan, ROC aut Chang Wan-Ting Behavioral Neuropharmacology Laboratory, Institute of Behavioral Medicine, National Cheng Kung University College of Medicine, 1 University Road, 70101, Tainan, Taiwan, ROC aut Cherng Chianfang Department of Health Psychology, Chang Jung Christian University, 71101, Tainan, Taiwan, ROC aut Kao Gour-Shenq Institute of Basic Medical Sciences, National Cheng Kung University College of Medicine, 70101, Tainan, Taiwan, ROC aut Yu Lung Institute of Basic Medical Sciences, National Cheng Kung University College of Medicine, 70101, Tainan, Taiwan, ROC aut Journal of Neural Transmission Springer Vienna 118/11(2011-11-01), 1559-1569 0300-9564 118:11<1559 2011 118 702 https://doi.org/10.1007/s00702-011-0645-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00702-011-0645-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chuang Jia-Ying Institute of Basic Medical Sciences, National Cheng Kung University College of Medicine, 70101, Tainan, Taiwan, ROC aut NATIONALLICENCE 700 1- Chang Wan-Ting Behavioral Neuropharmacology Laboratory, Institute of Behavioral Medicine, National Cheng Kung University College of Medicine, 1 University Road, 70101, Tainan, Taiwan, ROC aut NATIONALLICENCE 700 1- Cherng Chianfang Department of Health Psychology, Chang Jung Christian University, 71101, Tainan, Taiwan, ROC aut NATIONALLICENCE 700 1- Kao Gour-Shenq Institute of Basic Medical Sciences, National Cheng Kung University College of Medicine, 70101, Tainan, Taiwan, ROC aut NATIONALLICENCE 700 1- Yu Lung Institute of Basic Medical Sciences, National Cheng Kung University College of Medicine, 70101, Tainan, Taiwan, ROC aut NATIONALLICENCE 773 0- Journal of Neural Transmission Springer Vienna 118/11(2011-11-01), 1559-1569 0300-9564 118:11<1559 2011 118 702 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer