caa a22 4500 445378190 CHVBK 20180317143009.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10393-011-0724-8 doi (NATIONALLICENCE)springer-10.1007/s10393-011-0724-8 Molecular Techniques in Ecohealth Research Toolkit: Facilitating Estimation of Aggregate Gastroenteritis Burden in an Irrigated Periurban Landscape [Elektronische Daten] [Ariuntuya Tserendorj, Alfredo Anceno, Eric Houpt, Crystal Icenhour, Orntipa Sethabutr, Carl Mason, Oleg Shipin] Assessment of microbial hazards associated with certain environmental matrices, livelihood strategies, and food handling practices are constrained by time-consuming conventional microbiological techniques that lead to health risk assessments of narrow geographic or time scope, often targeting very few pathogens. Health risk assessment based on one or few indicator organisms underestimates true disease burden due a number of coexisting causative pathogens. Here, we employed molecular techniques in a survey of Cryptosporidium parvum, Giardia lamblia, Campylobacter jejuni, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella spp., Shigella spp., Vibrio cholera, and Rotavirus A densities in canal water with respect to seasonality and spatial distribution of point-nonpoint pollution sources. Three irrigational canals stretching across nearly a 150-km2 periurban landscape, traditionally used for agricultural irrigation but function as vital part of municipal wastewater stabilization in recent years, were investigated. Compiled stochastic data (pathogen concentration, susceptible populations) and literature-obtained deterministic data (pathogen dose-response model parameter values) were used in estimating waterborne gastroenteritis burden. Exposure scenarios include swimming or fishing, consuming canal water-irrigated vegetables, and ingesting or inhaling water aerosols while working in canal water-irrigated fields. Estimated annual gastroenteritis burden due individual pathogens among the sampling points was −10.6log10 to −2.2log10 DALYs. Aggregated annual gastroenteritis burden due all the target pathogens per sampling point was −3.1log10 to −1.9log10 DALYs, far exceeding WHO acceptable limit of −6.0log10 DALYs. The present approach will facilitate the comprehensive collection of surface water microbiological baseline data and setting of benchmarks for interventions aimed at reducing microbial hazards in similar landscapes worldwide. International Association for Ecology and Health, 2011 molecular techniques nationallicence indicator organisms nationallicence waterborne gastroenteritis nationallicence disease burden nationallicence microbial health risks nationallicence tropical periurban landscape nationallicence Tserendorj Ariuntuya WHO Collaborating Center for Water Supply and Waste Disposal, Environmental Engineering and Management, Asian Institute of Technology, P.O. Box 4, 12120, Klong Luang, Pathumthani, Thailand aut Anceno Alfredo WHO Collaborating Center for Water Supply and Waste Disposal, Environmental Engineering and Management, Asian Institute of Technology, P.O. Box 4, 12120, Klong Luang, Pathumthani, Thailand aut Houpt Eric Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA, USA aut Icenhour Crystal Phthisis Diagnostics, 705 Dale Avenue, 22903, Charlottesville, VA, USA aut Sethabutr Orntipa Department of Enteric Diseases, Armed Forces Research Institute of Medical Science, Bangkok, Thailand aut Mason Carl Department of Enteric Diseases, Armed Forces Research Institute of Medical Science, Bangkok, Thailand aut Shipin Oleg WHO Collaborating Center for Water Supply and Waste Disposal, Environmental Engineering and Management, Asian Institute of Technology, P.O. Box 4, 12120, Klong Luang, Pathumthani, Thailand aut EcoHealth Springer-Verlag 8/3(2011-09-01), 349-364 1612-9202 8:3<349 2011 8 10393 https://doi.org/10.1007/s10393-011-0724-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10393-011-0724-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tserendorj Ariuntuya WHO Collaborating Center for Water Supply and Waste Disposal, Environmental Engineering and Management, Asian Institute of Technology, P.O. Box 4, 12120, Klong Luang, Pathumthani, Thailand aut NATIONALLICENCE 700 1- Anceno Alfredo WHO Collaborating Center for Water Supply and Waste Disposal, Environmental Engineering and Management, Asian Institute of Technology, P.O. Box 4, 12120, Klong Luang, Pathumthani, Thailand aut NATIONALLICENCE 700 1- Houpt Eric Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA, USA aut NATIONALLICENCE 700 1- Icenhour Crystal Phthisis Diagnostics, 705 Dale Avenue, 22903, Charlottesville, VA, USA aut NATIONALLICENCE 700 1- Sethabutr Orntipa Department of Enteric Diseases, Armed Forces Research Institute of Medical Science, Bangkok, Thailand aut NATIONALLICENCE 700 1- Mason Carl Department of Enteric Diseases, Armed Forces Research Institute of Medical Science, Bangkok, Thailand aut NATIONALLICENCE 700 1- Shipin Oleg WHO Collaborating Center for Water Supply and Waste Disposal, Environmental Engineering and Management, Asian Institute of Technology, P.O. Box 4, 12120, Klong Luang, Pathumthani, Thailand aut NATIONALLICENCE 773 0- EcoHealth Springer-Verlag 8/3(2011-09-01), 349-364 1612-9202 8:3<349 2011 8 10393 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer