caa a22 4500 445384298 CHVBK 20180317143027.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10021-010-9408-4 doi (NATIONALLICENCE)springer-10.1007/s10021-010-9408-4 Linking Microbial and Ecosystem Ecology Using Ecological Stoichiometry: A Synthesis of Conceptual and Empirical Approaches [Elektronische Daten] [E. Hall, F. Maixner, O. Franklin, H. Daims, A. Richter, T. Battin] Currently, one of the biggest challenges in microbial and ecosystem ecology is to develop conceptual models that organize the growing body of information on environmental microbiology into a clear mechanistic framework with a direct link to ecosystem processes. Doing so will enable development of testable hypotheses to better direct future research and increase understanding of key constraints on biogeochemical networks. Although the understanding of phenotypic and genotypic diversity of microorganisms in the environment is rapidly accumulating, how controls on microbial physiology ultimately affect biogeochemical fluxes remains poorly understood. We propose that insight into constraints on biogeochemical cycles can be achieved by a more rigorous evaluation of microbial community biomass composition within the context of ecological stoichiometry. Multiple recent studies have pointed to microbial biomass stoichiometry as an important determinant of when microorganisms retain or recycle mineral nutrients. We identify the relevant cellular components that most likely drive changes in microbial biomass stoichiometry by defining a conceptual model rooted in ecological stoichiometry. More importantly, we show how X-ray microanalysis (XRMA), nanoscale secondary ion mass spectroscopy (NanoSIMS), Raman microspectroscopy, and in situ hybridization techniques (for example, FISH) can be applied in concert to allow for direct empirical evaluation of the proposed conceptual framework. This approach links an important piece of the ecological literature, ecological stoichiometry, with the molecular front of the microbial revolution, in an attempt to provide new insight into how microbial physiology could constrain ecosystem processes. The Author(s), 2010 biogeochemistry nationallicence ecological stoichiometry nationallicence community structure nationallicence ecosystem processes nationallicence homeostasis nationallicence nutrient ratios nationallicence Raman nationallicence NanoSIMS nationallicence XRMA nationallicence Hall E. Department of Limnology, University of Vienna, Althanstrasse 14, 1090, Vienna, Austria aut Maixner F. Department of Microbial Ecology, University of Vienna, Althanstrasse 14, 1090, Vienna, Austria aut Franklin O. IIASA International Institute for Applied Systems Analysis, 2361, Laxenburg, Austria aut Daims H. Department of Microbial Ecology, University of Vienna, Althanstrasse 14, 1090, Vienna, Austria aut Richter A. Department of Chemical Ecology, Vienna Ecology Center, University of Vienna, Althanstrasse 14, 1090, Vienna, Austria aut Battin T. Department of Limnology, University of Vienna, Althanstrasse 14, 1090, Vienna, Austria aut Ecosystems Springer-Verlag 14/2(2011-03-01), 261-273 1432-9840 14:2<261 2011 14 10021 https://doi.org/10.1007/s10021-010-9408-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10021-010-9408-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hall E. Department of Limnology, University of Vienna, Althanstrasse 14, 1090, Vienna, Austria aut NATIONALLICENCE 700 1- Maixner F. Department of Microbial Ecology, University of Vienna, Althanstrasse 14, 1090, Vienna, Austria aut NATIONALLICENCE 700 1- Franklin O. IIASA International Institute for Applied Systems Analysis, 2361, Laxenburg, Austria aut NATIONALLICENCE 700 1- Daims H. Department of Microbial Ecology, University of Vienna, Althanstrasse 14, 1090, Vienna, Austria aut NATIONALLICENCE 700 1- Richter A. Department of Chemical Ecology, Vienna Ecology Center, University of Vienna, Althanstrasse 14, 1090, Vienna, Austria aut NATIONALLICENCE 700 1- Battin T. Department of Limnology, University of Vienna, Althanstrasse 14, 1090, Vienna, Austria aut NATIONALLICENCE 773 0- Ecosystems Springer-Verlag 14/2(2011-03-01), 261-273 1432-9840 14:2<261 2011 14 10021 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer