caa a22 4500 445384611 CHVBK 20180317143028.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10924-010-0254-4 doi (NATIONALLICENCE)springer-10.1007/s10924-010-0254-4 Fluorescence Verses Radioactivity Labeling for Lab-Scale Investigation of the Fate of Water-Soluble Polymers in Wastewater Treatment Plants [Elektronische Daten] [Anne-Catherine Bénard, Vincent Darcos, Christian Drakides, Claude Casellas, Jean Coudane, Michel Vert] Water-soluble synthetic polymers are extensively used in cosmetics, detergents and paints. Many end up in wastewater and, later on, in wastewater-treatment plants. In order to gain an insight into their fate in such plants, fluorescence and radioactivity labelings were compared using a lab-scale reactor designed to mimic industrial conditions. Two fermentation media were considered, namely a mixture of E402 and E204 micro-organisms and an activated sludge collected from a water-treatment plant located in the south of France. A sample of low molar mass commercial poly(acrylic acid) (PAA) was labeled by radioactivity with tritium and by coupling the 6-aminofluorescein fluorescent dye. Labeled PAA-containing sludges were allowed to ferment. To monitor the fate of the polymers, aliquots of the fermented mixtures were withdrawn at selected times and centrifuged. Liquid and solid phases were analyzed by scintigraphy or UV spectrometry, depending of the labeling techniques. Both techniques led to similar distributions, c.a. 75% in the supernatant and 25% in the solid phase. Distributions remained constant during the biological tests. There was no degradation of the commercial PAA after aqueous size exclusion chromatography (SEC), in agreement with literature. These features showed that fluorescence-labeling can be used instead of the complex and expensive radiolabeling. The validated fluorescence-based method was then applied to a linear poly(acrylic acid) synthesized by ATRP and labeled with 6-aminofluorescein. There was no significant difference between the commercial and the linear poly(acrylic acid)s. In contrast, a linear PAA with 5% of tert-butyl ester repeating units was predominantly found in the solid phase although adsorption or absorption by micro-organisms could not be demonstrated. The method based on fluorescence labeling should be applicable to other water soluble polymers provided that the dye remains attached to the polymer as it was the case for the studied poly(acrylic acid)s. Springer Science+Business Media, LLC, 2010 Water-soluble polymers nationallicence Degradation nationallicence Wastewater treatment nationallicence Radioactivity labeling nationallicence Fluorescence labeling nationallicence Bénard Anne-Catherine Max Mousseron Institute of Biomolecules, UMR CNRS 5247, Faculty of Pharmacy, University of Montpellier 1, University of Montpellier 2, 15 Avenue Charles Flahault, BP 14491, 34093, Montpellier cedex 5, France aut Darcos Vincent Max Mousseron Institute of Biomolecules, UMR CNRS 5247, Faculty of Pharmacy, University of Montpellier 1, University of Montpellier 2, 15 Avenue Charles Flahault, BP 14491, 34093, Montpellier cedex 5, France aut Drakides Christian UMR CNRS 5569, Hydrosciences Montpellier, Faculty of Pharmacy, University of Montpellier 1, 15 Avenue Charles Flahault, BP 14 491, 34093, Montpellier Cedex 5, France aut Casellas Claude UMR CNRS 5569, Hydrosciences Montpellier, Faculty of Pharmacy, University of Montpellier 1, 15 Avenue Charles Flahault, BP 14 491, 34093, Montpellier Cedex 5, France aut Coudane Jean Max Mousseron Institute of Biomolecules, UMR CNRS 5247, Faculty of Pharmacy, University of Montpellier 1, University of Montpellier 2, 15 Avenue Charles Flahault, BP 14491, 34093, Montpellier cedex 5, France aut Vert Michel Max Mousseron Institute of Biomolecules, UMR CNRS 5247, Faculty of Pharmacy, University of Montpellier 1, University of Montpellier 2, 15 Avenue Charles Flahault, BP 14491, 34093, Montpellier cedex 5, France aut Journal of Polymers and the Environment Springer US; http://www.springer-ny.com 19/1(2011-03-01), 40-48 1566-2543 19:1<40 2011 19 10924 https://doi.org/10.1007/s10924-010-0254-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10924-010-0254-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bénard Anne-Catherine Max Mousseron Institute of Biomolecules, UMR CNRS 5247, Faculty of Pharmacy, University of Montpellier 1, University of Montpellier 2, 15 Avenue Charles Flahault, BP 14491, 34093, Montpellier cedex 5, France aut NATIONALLICENCE 700 1- Darcos Vincent Max Mousseron Institute of Biomolecules, UMR CNRS 5247, Faculty of Pharmacy, University of Montpellier 1, University of Montpellier 2, 15 Avenue Charles Flahault, BP 14491, 34093, Montpellier cedex 5, France aut NATIONALLICENCE 700 1- Drakides Christian UMR CNRS 5569, Hydrosciences Montpellier, Faculty of Pharmacy, University of Montpellier 1, 15 Avenue Charles Flahault, BP 14 491, 34093, Montpellier Cedex 5, France aut NATIONALLICENCE 700 1- Casellas Claude UMR CNRS 5569, Hydrosciences Montpellier, Faculty of Pharmacy, University of Montpellier 1, 15 Avenue Charles Flahault, BP 14 491, 34093, Montpellier Cedex 5, France aut NATIONALLICENCE 700 1- Coudane Jean Max Mousseron Institute of Biomolecules, UMR CNRS 5247, Faculty of Pharmacy, University of Montpellier 1, University of Montpellier 2, 15 Avenue Charles Flahault, BP 14491, 34093, Montpellier cedex 5, France aut NATIONALLICENCE 700 1- Vert Michel Max Mousseron Institute of Biomolecules, UMR CNRS 5247, Faculty of Pharmacy, University of Montpellier 1, University of Montpellier 2, 15 Avenue Charles Flahault, BP 14491, 34093, Montpellier cedex 5, France aut NATIONALLICENCE 773 0- Journal of Polymers and the Environment Springer US; http://www.springer-ny.com 19/1(2011-03-01), 40-48 1566-2543 19:1<40 2011 19 10924 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer