caa a22 4500 445384646 CHVBK 20180317143028.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10924-010-0262-4 doi (NATIONALLICENCE)springer-10.1007/s10924-010-0262-4 Evaluation of Cyto- and Genotoxicity of Poly(lactide-co-glycolide) Nanoparticles [Elektronische Daten] [Renata de Lima, Anderson do Espirito Santo Pereira, Raquel Porto, Leonardo Fraceto] This work reports on an analysis of the cyto- and genotoxicity of poly(lactide-co-glycolide) polymer nanoparticles, in an attempt to evaluate their mutagenic effects. Fibroblast (3T3) and human lymphocyte cell cultures were exposed to solutions containing three different concentrations of nanoparticles (5.4, 54 and 540μg/mL, polymer mass/volume of solution). The nanoparticles were characterized in terms of their hydrodynamic diameters, zeta potentials and polydispersity indices. The morphology of the particles was determined by atomic force microscopy. The PLGA nanospheres presented a size of 95nm, a zeta potential of −20mV and a spherical morphology. Cellular viability assays using fibroblast cells showed no significant alterations compared with the negative control. A cytogenetic analysis of human lymphocyte cells showed no significant changes in the mitotic index in relation to the control, indicating that in the concentration range tested, the particles used in the experimental models did not present cyto- or genotoxicity. For the tests conducted in this work we can conclude that biodegradable and biocompatible PLGA nanospheres are not toxic in the cell cultures tested (fibroblast and lymphocyte cells) and in the range of concentrations employed. The results provide new information concerning the toxic effects of particles produced using PLGA. Springer Science+Business Media, LLC, 2010 Polymeric nanoparticles nationallicence Cytotoxicity nationallicence Genotoxicity nationallicence Environmental toxicity nationallicence de Lima Renata Department of Biotechnology, University of Sorocaba, Sorocaba, SP, Brazil aut do Espirito Santo Pereira Anderson Department of Biotechnology, University of Sorocaba, Sorocaba, SP, Brazil aut Porto Raquel Department of Biotechnology, University of Sorocaba, Sorocaba, SP, Brazil aut Fraceto Leonardo Department of Environmental Engineering, São Paulo State University Júlio de Mesquita Filho, Campus Sorocaba, Av. Três de Março, 511, 18087-180, Sorocaba, SP, Brazil aut Journal of Polymers and the Environment Springer US; http://www.springer-ny.com 19/1(2011-03-01), 196-202 1566-2543 19:1<196 2011 19 10924 https://doi.org/10.1007/s10924-010-0262-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10924-010-0262-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Lima Renata Department of Biotechnology, University of Sorocaba, Sorocaba, SP, Brazil aut NATIONALLICENCE 700 1- do Espirito Santo Pereira Anderson Department of Biotechnology, University of Sorocaba, Sorocaba, SP, Brazil aut NATIONALLICENCE 700 1- Porto Raquel Department of Biotechnology, University of Sorocaba, Sorocaba, SP, Brazil aut NATIONALLICENCE 700 1- Fraceto Leonardo Department of Environmental Engineering, São Paulo State University Júlio de Mesquita Filho, Campus Sorocaba, Av. Três de Março, 511, 18087-180, Sorocaba, SP, Brazil aut NATIONALLICENCE 773 0- Journal of Polymers and the Environment Springer US; http://www.springer-ny.com 19/1(2011-03-01), 196-202 1566-2543 19:1<196 2011 19 10924 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer