caa a22 4500 445384751 CHVBK 20180317143029.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10924-011-0326-0 doi (NATIONALLICENCE)springer-10.1007/s10924-011-0326-0 1,3-Propanediol Production Potential by a Locally Isolated Strain of Klebsiella Pneumoniae in Comparison to Clostridium Beijerinckii NRRL B593 from Waste Glycerol [Elektronische Daten] [Mine Gungormusler, Cagdas Gonen, Nuri Azbar] The fermentative production of 1,3-propanediol (1,3-PDO) by Klebsiella pneumoniae under different initial substrate concentrations (between 5 and 110g/L) was investigated. It was found that glycerol was almost 100% utilized and 1,3-PDO production increased up to 20g/L of influent substrate concentration, but there was a significant decrease in both glycerol consumption and 1,3-PDO production at substrate concentrations exceeding 20g/L. Furthermore, pH control was essential, and a lack of pH control negatively effects of 1,3-PDO production. In the second part of the study, two microorganisms, namely Clostridium beijerinckii NRRL B593 and K. pneumoniae were comparatively studied in terms of their 1,3-PDO productivity under pH controlled conditions. Higher 1,3-PDO production was achieved under pH controlled fermentation conditions (pH=7) for both microorganisms. Even though the two microorganisms had almost the same 1,3-PDO yield (0.60mol/mol for C. beijerinckii, 0.61mol/mol for K. pneumoniae) at the end of fermentation period, K. pneumoniae completed the 1,3-PDO production in one-third of the time (t=8h with a productivity of 1.34g/L/h) than C. beijerinckii (t=24h). The results of this study clearly indicated that a substrate inhibition is a challenge that needs to be studied further for higher productivities. Springer Science+Business Media, LLC, 2011 1,3-Propanediol nationallicence Klebsiella pneumoniae nationallicence Biodiesel derived raw glycerol nationallicence Clostridium beijerinckii NRRL B593 nationallicence Gungormusler Mine Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey aut Gonen Cagdas Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey aut Azbar Nuri Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey aut Journal of Polymers and the Environment Springer US; http://www.springer-ny.com 19/3(2011-09-01), 812-817 1566-2543 19:3<812 2011 19 10924 https://doi.org/10.1007/s10924-011-0326-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10924-011-0326-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gungormusler Mine Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey aut NATIONALLICENCE 700 1- Gonen Cagdas Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey aut NATIONALLICENCE 700 1- Azbar Nuri Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey aut NATIONALLICENCE 773 0- Journal of Polymers and the Environment Springer US; http://www.springer-ny.com 19/3(2011-09-01), 812-817 1566-2543 19:3<812 2011 19 10924 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer