caa a22 4500 445384999 CHVBK 20180317143029.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10924-011-0324-2 doi (NATIONALLICENCE)springer-10.1007/s10924-011-0324-2 Kinetic Study of Biopolymer (PHB) Synthesis in Alcaligenes sp. in Submerged Fermentation Process Using TEM [Elektronische Daten] [Abhishek Tripathi, S. Srivastava] Poly-β-hydroxybuyrate (PHB) is a carbon—energy storage material which is accumulated as intracellular granule in variety of microorganism under nutrient starved conditions. Solid PHB is a biodegradable thermoplastic polymer and is utilizable in various ways similar to many conventional plastics. Ralstonia eutropha (Alcaligenes sp.), a gram negative bacteria accumulates PHB as insoluble granules inside the cells when nutrients other than carbon are limited. In this report effort has been made to analyze PHB granule synthesis inside Alcaligenes sp. NCIM 5085 by transmission electron microscopy and qualitative estimation of PHB was carried out by fourier transform infrared spectroscopy which provide better precision compared to other conventional techniques previously applied for PHB determination. Maximum PHB concentration of 2.20±0.40g/L and cell biomass of 3.42±0.20g/L was obtained after 48.0h of fermentation. Leudking-Piret equation deduced mixed growth associated product formation which varies from earlier reports. Springer Science+Business Media, LLC, 2011 Poly-hydroxybuyrate nationallicence Transmission electron microscopy (TEM) nationallicence Fourier Transform infrared spectroscopy (FTIR) nationallicence Leudking -Piret equation nationallicence Tripathi Abhishek School of Biochemical Engineering, Institute of Technology, Banaras Hindu University, 221005, Varanasi, UP, India aut Srivastava S. School of Biochemical Engineering, Institute of Technology, Banaras Hindu University, 221005, Varanasi, UP, India aut Journal of Polymers and the Environment Springer US; http://www.springer-ny.com 19/3(2011-09-01), 732-738 1566-2543 19:3<732 2011 19 10924 https://doi.org/10.1007/s10924-011-0324-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10924-011-0324-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tripathi Abhishek School of Biochemical Engineering, Institute of Technology, Banaras Hindu University, 221005, Varanasi, UP, India aut NATIONALLICENCE 700 1- Srivastava S. School of Biochemical Engineering, Institute of Technology, Banaras Hindu University, 221005, Varanasi, UP, India aut NATIONALLICENCE 773 0- Journal of Polymers and the Environment Springer US; http://www.springer-ny.com 19/3(2011-09-01), 732-738 1566-2543 19:3<732 2011 19 10924 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer