caa a22 4500 445385081 CHVBK 20180317143030.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10924-011-0346-9 doi (NATIONALLICENCE)springer-10.1007/s10924-011-0346-9 Gene Cloning and Characterization of a Poly( l -Lactic Acid) Depolymerase from Pseudomonas sp. Strain DS04-T [Elektronische Daten] [Zhan-Yong Wang, Fan Li, Zi-Qi Guo, Yan Wang, Shan Chen] A gene encoding a poly(l-lactic acid) (PLA) depolymerase from Pseudomonas sp. strain DS04-T was cloned and overexpressed in Escherichia coli. The recombinant PLA depolymerase with a molecular weight of 19.2kDa was purified to homogeneity. The optimum pH and temperature of the PLA depolymerase are 8.5 and 60°C, respectively. K+, Ca2+ and Ni2+ enhance the enzyme activity, while Na+, Zn2+, Mg2+, Cu2+, Fe2+, Mn2+ and Co2+ inhibit it. The inhibition of different chemicals on the PLA depolymerase activity were examined, in which EDTA was found to have a significantly inhibitory effect. The main degradation product of the depolymerase is identified as lactic acid monomer by mass spectrometric analysis. Physicochemical properties, substrate specificity and sequence analysis indicated that PME is a new type of PLA depolymerase. Springer Science+Business Media, LLC, 2011 Poly( l -lactic acid) nationallicence Depolymerase nationallicence Pseudomonas sp. strain DS04-T nationallicence Recombinant nationallicence Wang Zhan-Yong School of Life Sciences, Northeast Normal University, 130024, Changchun, People's Republic of China aut Li Fan School of Life Sciences, Northeast Normal University, 130024, Changchun, People's Republic of China aut Guo Zi-Qi School of Life Sciences, Northeast Normal University, 130024, Changchun, People's Republic of China aut Wang Yan School of Life Sciences, Northeast Normal University, 130024, Changchun, People's Republic of China aut Chen Shan School of Life Sciences, Northeast Normal University, 130024, Changchun, People's Republic of China aut Journal of Polymers and the Environment Springer US; http://www.springer-ny.com 19/4(2011-12-01), 827-833 1566-2543 19:4<827 2011 19 10924 https://doi.org/10.1007/s10924-011-0346-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10924-011-0346-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Zhan-Yong School of Life Sciences, Northeast Normal University, 130024, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Li Fan School of Life Sciences, Northeast Normal University, 130024, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Guo Zi-Qi School of Life Sciences, Northeast Normal University, 130024, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Wang Yan School of Life Sciences, Northeast Normal University, 130024, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Chen Shan School of Life Sciences, Northeast Normal University, 130024, Changchun, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Polymers and the Environment Springer US; http://www.springer-ny.com 19/4(2011-12-01), 827-833 1566-2543 19:4<827 2011 19 10924 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer