caa a22 4500 445389583 CHVBK 20180317143042.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00049-011-0082-6 doi (NATIONALLICENCE)springer-10.1007/s00049-011-0082-6 Impact of hydroxylated and non-hydroxylated aliphatic glucosinolates in Arabidopsis thaliana crosses on plant resistance against a generalist and a specialist herbivore [Elektronische Daten] [Franziska Rohr, Christian Ulrichs, Monika Schreiner, Chau Nguyen, Inga Mewis] Glucosinolates (GSs) are part of a two-component defence system, characteristic for the Brassicales, including the model species Arabidopsis thaliana (L.) Heynh. The defence activity of GSs is associated with different side chain structures. The AOP genes are central in side-chain modification. AOP2 mediates formation of alkenyl GS from a methylsulfinyl precursor, whereas AOP3 catalyzes production of hydroxy-alkyl GSs from the same precursor. Although several studies have assessed the role of GSs in plant defence, the function of specific aliphatic GSs in plant defence is still not clarified. Structural different GSs may influence insect herbivores differentially. We created a set of plant lines derived of a cross between two A. thaliana accessions, Gie-0 × Sap-0, which dominantly accumulate either 3-methylsulfinylpropyl GS or 3-hydroxypropyl GS. The generalist Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) and the crucifer-specialist Pieris brassicae (L.) (Lepidoptera: Pieridae) were used as model insects, to study effects of individual aliphatic GSs on lepidopteran herbivores with a different feeding specialization. However, the experiments revealed that weight gain of S. exigua and P. brassicae third and fourth-larval instars was similar on both chemotypes. But leaf consumption of the generalist was higher on 3-methylsulfinylpropyl-producing lines with low GS levels (23.2μmolg−1) than on 3-hydroxypropyl-producing lines that contained a more than twofold higher amount of GSs (60μmolg−1). In contrast, no differential effects of non-hydroxylated and hydroxylated GSs were found on the specialist P. brassicae. Our study indicates that there is no simple relationship between GS content and insect responses. Springer Basel AG, 2011 Glucosinolate nationallicence Aliphatic side chain nationallicence Pieridae nationallicence Noctuidae nationallicence Chemical defence nationallicence Insect performance nationallicence Rohr Franziska Division Urban Plant Ecophysiology, Faculty for Agriculture and Horticulture, Humboldt Universität zu Berlin, Lentzeallee 55, 14195, Berlin, Germany aut Ulrichs Christian Division Urban Plant Ecophysiology, Faculty for Agriculture and Horticulture, Humboldt Universität zu Berlin, Lentzeallee 55, 14195, Berlin, Germany aut Schreiner Monika Leibniz-Institute of Vegetable and Ornamental Crops Großbeeren/Erfurt e.V, Theodor-Echtermeyer-Weg 1, 14979, Großbeeren, Germany aut Nguyen Chau Division Urban Plant Ecophysiology, Faculty for Agriculture and Horticulture, Humboldt Universität zu Berlin, Lentzeallee 55, 14195, Berlin, Germany aut Mewis Inga Division Urban Plant Ecophysiology, Faculty for Agriculture and Horticulture, Humboldt Universität zu Berlin, Lentzeallee 55, 14195, Berlin, Germany aut Chemoecology SP Birkhäuser Verlag Basel 21/3(2011-09-01), 171-180 0937-7409 21:3<171 2011 21 49 https://doi.org/10.1007/s00049-011-0082-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00049-011-0082-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rohr Franziska Division Urban Plant Ecophysiology, Faculty for Agriculture and Horticulture, Humboldt Universität zu Berlin, Lentzeallee 55, 14195, Berlin, Germany aut NATIONALLICENCE 700 1- Ulrichs Christian Division Urban Plant Ecophysiology, Faculty for Agriculture and Horticulture, Humboldt Universität zu Berlin, Lentzeallee 55, 14195, Berlin, Germany aut NATIONALLICENCE 700 1- Schreiner Monika Leibniz-Institute of Vegetable and Ornamental Crops Großbeeren/Erfurt e.V, Theodor-Echtermeyer-Weg 1, 14979, Großbeeren, Germany aut NATIONALLICENCE 700 1- Nguyen Chau Division Urban Plant Ecophysiology, Faculty for Agriculture and Horticulture, Humboldt Universität zu Berlin, Lentzeallee 55, 14195, Berlin, Germany aut NATIONALLICENCE 700 1- Mewis Inga Division Urban Plant Ecophysiology, Faculty for Agriculture and Horticulture, Humboldt Universität zu Berlin, Lentzeallee 55, 14195, Berlin, Germany aut NATIONALLICENCE 773 0- Chemoecology SP Birkhäuser Verlag Basel 21/3(2011-09-01), 171-180 0937-7409 21:3<171 2011 21 49 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer