caa a22 4500 445389613 CHVBK 20180317143042.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00049-011-0073-7 doi (NATIONALLICENCE)springer-10.1007/s00049-011-0073-7 Effect of nitrogen fertilization on caffeine production in coffee ( Coffea arabica ) [Elektronische Daten] [David Gonthier, Jason Witter, Alison Spongberg, Stacy Philpott] Nitrogen (N) based secondary metabolite production is thought to be costly to plants because N is required for growth, as well as, the synthesis of these compounds. Therefore, variation in N availability may result in variation in N-based secondary metabolite production. Here, we determine the effect of N fertilization on caffeine (N-based alkaloid) production in coffee (Coffea arabica) seedlings. A growth chamber experiment was performed with three N treatments applied to seedlings. N fertilization increased plant growth, leaf biomass, and plant N. Caffeine concentration in phloem exudates was greater in high-N fertilized plants relative to intermediate- and low-N plants. However, leaf, stem, root, and total overall caffeine concentration and content did not differ across N treatments. These results suggest caffeine in coffee is strongly regulated by genetic factors, and environment is likely less important to caffeine phenotype. This is among the first studies to investigate the effect of N fertilization on caffeine within the phloem, which has important implications for herbivores that are sensitive to caffeine and plant N and feed from the phloem of coffee. Springer Basel AG, 2011 Secondary metabolites nationallicence Methylxanthine nationallicence Alkaloid nationallicence Resource availability nationallicence Nitrogen nationallicence Chemical defense nationallicence Gonthier David Department of Environmental Sciences, University of Toledo, 2801 W. Bancroft Rd., Mail Stop 604, 43606, Toledo, OH, USA aut Witter Jason Department of Environmental Sciences, University of Toledo, 2801 W. Bancroft Rd., Mail Stop 604, 43606, Toledo, OH, USA aut Spongberg Alison Department of Environmental Sciences, University of Toledo, 2801 W. Bancroft Rd., Mail Stop 604, 43606, Toledo, OH, USA aut Philpott Stacy Department of Environmental Sciences, University of Toledo, 2801 W. Bancroft Rd., Mail Stop 604, 43606, Toledo, OH, USA aut Chemoecology SP Birkhäuser Verlag Basel 21/3(2011-09-01), 123-130 0937-7409 21:3<123 2011 21 49 https://doi.org/10.1007/s00049-011-0073-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00049-011-0073-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gonthier David Department of Environmental Sciences, University of Toledo, 2801 W. Bancroft Rd., Mail Stop 604, 43606, Toledo, OH, USA aut NATIONALLICENCE 700 1- Witter Jason Department of Environmental Sciences, University of Toledo, 2801 W. Bancroft Rd., Mail Stop 604, 43606, Toledo, OH, USA aut NATIONALLICENCE 700 1- Spongberg Alison Department of Environmental Sciences, University of Toledo, 2801 W. Bancroft Rd., Mail Stop 604, 43606, Toledo, OH, USA aut NATIONALLICENCE 700 1- Philpott Stacy Department of Environmental Sciences, University of Toledo, 2801 W. Bancroft Rd., Mail Stop 604, 43606, Toledo, OH, USA aut NATIONALLICENCE 773 0- Chemoecology SP Birkhäuser Verlag Basel 21/3(2011-09-01), 123-130 0937-7409 21:3<123 2011 21 49 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer