caa a22 4500 445389745 CHVBK 20180317143042.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00049-011-0068-4 doi (NATIONALLICENCE)springer-10.1007/s00049-011-0068-4 Evidence for the stratification of hydrocarbons in the epicuticular wax layer of female Megacyllene robiniae (Coleoptera: Cerambycidae) [Elektronische Daten] [Gabriel Hughes, Annie Spikes, Jeffrey Holland, Matthew Ginzel] Contact pheromones mediate mate recognition and play important roles in mating systems of longhorned beetles (Coleoptera: Cerambycidae). One common bioassay of contact chemoreception in cerambycids involves presenting a freeze-killed female to a male in a Petri dish arena. If the male attempts to mate with the female carcass, it confirms that mate recognition signals are present and intact and behavior is not involved. Cuticular hydrocarbons are then stripped from the female with successive solvent washes, rendering her unattractive to males and also resulting in a crude extract containing the cuticular hydrocarbons. To test the bioactivity of the crude extract, the same female is then treated with the extract and presented again to the male. Males of some species, including Megacyllene robiniae (Förster), respond less readily to reconstituted females than to those same beetles before they were solvent-extracted. In the present study, we test the hypothesis that the contact pheromone of M. robiniae, Z9:C25, exists as a layer on the surface of the epicuticle. We used solid phase microextraction (SPME) to sample cuticular hydrocarbons of female beetles after they were freeze-killed, solvent washed, and treated with crude cuticular extracts. We found that extracting cuticular hydrocarbons from females and applying the resulting crude extract back onto the solvent-washed cadaver scrambles the wax layer and decreases the abundance of the contact pheromone presented on the surface of the insect. Springer Basel AG, 2011 Solid phase microextraction nationallicence Contact pheromone nationallicence Cuticular hydrocarbon nationallicence Hughes Gabriel Department of Entomology, Purdue University, 901 W. State Street, 47907-2089, West Lafayette, IN, USA aut Spikes Annie Department of Entomology, Purdue University, 901 W. State Street, 47907-2089, West Lafayette, IN, USA aut Holland Jeffrey Department of Entomology, Purdue University, 901 W. State Street, 47907-2089, West Lafayette, IN, USA aut Ginzel Matthew Department of Entomology, Purdue University, 901 W. State Street, 47907-2089, West Lafayette, IN, USA aut Chemoecology SP Birkhäuser Verlag Basel 21/2(2011-06-01), 99-105 0937-7409 21:2<99 2011 21 49 https://doi.org/10.1007/s00049-011-0068-4 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00049-011-0068-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hughes Gabriel Department of Entomology, Purdue University, 901 W. State Street, 47907-2089, West Lafayette, IN, USA aut NATIONALLICENCE 700 1- Spikes Annie Department of Entomology, Purdue University, 901 W. State Street, 47907-2089, West Lafayette, IN, USA aut NATIONALLICENCE 700 1- Holland Jeffrey Department of Entomology, Purdue University, 901 W. State Street, 47907-2089, West Lafayette, IN, USA aut NATIONALLICENCE 700 1- Ginzel Matthew Department of Entomology, Purdue University, 901 W. State Street, 47907-2089, West Lafayette, IN, USA aut NATIONALLICENCE 773 0- Chemoecology SP Birkhäuser Verlag Basel 21/2(2011-06-01), 99-105 0937-7409 21:2<99 2011 21 49 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer