caa a22 4500 445389796 CHVBK 20180317143043.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00049-011-0069-3 doi (NATIONALLICENCE)springer-10.1007/s00049-011-0069-3 Sex pheromone composition and reproductive isolation in two Busseola species (Lepidoptera: Noctuidae) in Kenya [Elektronische Daten] [A.-E. Félix, P.-A. Calatayud, B. Le Ru, J.-F. Silvain, B. Frérot] Closely related species provide an interesting and useful model to understand mate communication diversification. Pre-mating isolation is the result of selection processes and is achieved by utilization of different sex pheromones, but also by different ecological and life traits such as geographic distribution, host-plant specialization and allochrony of sexual behavior. Here, we report sex pheromone identifications and pre-mating isolation of two closely related species of stemborers: Busseola segeta and the yet undescribed Busseola nov. sp., developing on different host-plants and in different geographical locations in Kenya. GC-MS analyses showed that the two species produced the same pheromone components, but in slight different ratios. The respective synthetic pheromone mixtures tested in the field were attractive for males of each species. The low inter-specific variation in the pheromone mixture would allow possible interbreeding in case of accidental introduction of both species in the same environment. Under laboratory conditions, allochrony of the mating period is total and might prevent interbreeding if confirmed in natural conditions. This point would ensure the reproductive isolation between the two species in case of modifications of ecological factors. This study and the chemical structures of the identified pheromone components clearly demonstrate that these Busseola species are closely related although they differ in morphology. Springer Basel AG, 2011 Busseola segeta nationallicence Busseola nov. sp nationallicence Solid phase micro extraction nationallicence Gas-chromatography linked to mass spectrometry nationallicence Field trapping nationallicence Reproductive isolation nationallicence Félix A.-E INRA, UMR PISC 1272, Route de St. Cyr, 78026, Versailles Cedex, France aut Calatayud P.-A IRD, UR072, Laboratoire Evolution, Génomes et Spéciation (LEGS), UPR 9034, CNRS, 91198, Gif-sur-Yvette cedex, France aut Le Ru B. IRD, UR072, Laboratoire Evolution, Génomes et Spéciation (LEGS), UPR 9034, CNRS, 91198, Gif-sur-Yvette cedex, France aut Silvain J.-F IRD, UR072, Laboratoire Evolution, Génomes et Spéciation (LEGS), UPR 9034, CNRS, 91198, Gif-sur-Yvette cedex, France aut Frérot B. INRA, UMR PISC 1272, Route de St. Cyr, 78026, Versailles Cedex, France aut Chemoecology SP Birkhäuser Verlag Basel 21/2(2011-06-01), 107-111 0937-7409 21:2<107 2011 21 49 https://doi.org/10.1007/s00049-011-0069-3 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00049-011-0069-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Félix A.-E INRA, UMR PISC 1272, Route de St. Cyr, 78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Calatayud P.-A IRD, UR072, Laboratoire Evolution, Génomes et Spéciation (LEGS), UPR 9034, CNRS, 91198, Gif-sur-Yvette cedex, France aut NATIONALLICENCE 700 1- Le Ru B. IRD, UR072, Laboratoire Evolution, Génomes et Spéciation (LEGS), UPR 9034, CNRS, 91198, Gif-sur-Yvette cedex, France aut NATIONALLICENCE 700 1- Silvain J.-F IRD, UR072, Laboratoire Evolution, Génomes et Spéciation (LEGS), UPR 9034, CNRS, 91198, Gif-sur-Yvette cedex, France aut NATIONALLICENCE 700 1- Frérot B. INRA, UMR PISC 1272, Route de St. Cyr, 78026, Versailles Cedex, France aut NATIONALLICENCE 773 0- Chemoecology SP Birkhäuser Verlag Basel 21/2(2011-06-01), 107-111 0937-7409 21:2<107 2011 21 49 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer