caa a22 4500 445390190 CHVBK 20180317143044.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10327-010-0273-y doi (NATIONALLICENCE)springer-10.1007/s10327-010-0273-y Genetic diversity and pathogenicity of cucurbit-associated Acidovorax [Elektronische Daten] [Yoshiyuki Makizumi, Mitsuru Igarashi, Kiyotaka Gotoh, Kazunori Murao, Momoe Yamamoto, Nutthawoot Udonsri, Hirokazu Ochiai, Petcharat Thummabenjapone, Hisatoshi Kaku] Bacterial fruit blotch of cucurbits is a destructive disease caused by Acidovorax avenae subsp. citrulli, which is a typical seedborne pathogen. In seed health testing for this disease, we have detected many strains of Acidovorax with some differences from A. avenae subsp. citrulli. Their 16S rRNA sequences were divided into six types. The most common sequence was completely consistent with that of A. avenae subsp. avenae originally isolated from rice. The other sequences were over 99% similar but not identical to those of A. avenae subsp. avenae and A. avenae subsp. citrulli. Some commercialized antibodies against A. avenae subsp. citrulli reacted with several of these strains. Some of these strains incited yellow spots or brownish water-soaked lesions mainly on young true leaves of cucumber and squash after spray inoculation. Histological observations showed that these strains entered the leaf tissues of cucurbit plants through stomata and multiplied in the intercellular spaces of parenchymatous tissues as well as in the vascular tissues. The amount of bacterial multiplication and spread in the tissues differed among the strains, presumably reflecting their ability to induce symptoms. These isolated strains are therefore different from A. avenae subsp. citrulli, and their potential threat to the cultivation of cucurbits is lower than that of A. avenae subsp. citrulli. The Phytopathological Society of Japan and Springer, 2010 Acidovorax nationallicence Bacterial fruit blotch (BFB) nationallicence Seedborne pathogen nationallicence 16S rRNA sequence nationallicence Pathogenicity nationallicence Seed health test nationallicence Makizumi Yoshiyuki Sakata Seed Corporation, 224-0041, Yokohama, Japan aut Igarashi Mitsuru Sakata Seed Corporation, 224-0041, Yokohama, Japan aut Gotoh Kiyotaka Sakata Seed Corporation, 224-0041, Yokohama, Japan aut Murao Kazunori Sakata Seed Corporation, 224-0041, Yokohama, Japan aut Yamamoto Momoe Sakata Seed Corporation, 224-0041, Yokohama, Japan aut Udonsri Nutthawoot Sakata Siam Seed Co., Ltd., 40000, Khon Kaen, Thailand aut Ochiai Hirokazu National Institute of Agrobiological Sciences, 305-8602, Tsukuba, Japan aut Thummabenjapone Petcharat Faculty of Agriculture, Khon Kaen University, 40002, Khon Kaen, Thailand aut Kaku Hisatoshi Sakata Seed Corporation, 224-0041, Yokohama, Japan aut Journal of General Plant Pathology Springer Japan 77/1(2011-01-01), 24-32 1345-2630 77:1<24 2011 77 10327 https://doi.org/10.1007/s10327-010-0273-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-010-0273-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Makizumi Yoshiyuki Sakata Seed Corporation, 224-0041, Yokohama, Japan aut NATIONALLICENCE 700 1- Igarashi Mitsuru Sakata Seed Corporation, 224-0041, Yokohama, Japan aut NATIONALLICENCE 700 1- Gotoh Kiyotaka Sakata Seed Corporation, 224-0041, Yokohama, Japan aut NATIONALLICENCE 700 1- Murao Kazunori Sakata Seed Corporation, 224-0041, Yokohama, Japan aut NATIONALLICENCE 700 1- Yamamoto Momoe Sakata Seed Corporation, 224-0041, Yokohama, Japan aut NATIONALLICENCE 700 1- Udonsri Nutthawoot Sakata Siam Seed Co., Ltd., 40000, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Ochiai Hirokazu National Institute of Agrobiological Sciences, 305-8602, Tsukuba, Japan aut NATIONALLICENCE 700 1- Thummabenjapone Petcharat Faculty of Agriculture, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Kaku Hisatoshi Sakata Seed Corporation, 224-0041, Yokohama, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/1(2011-01-01), 24-32 1345-2630 77:1<24 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer