caa a22 4500 445390263 CHVBK 20180317143044.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10327-010-0282-x doi (NATIONALLICENCE)springer-10.1007/s10327-010-0282-x Verticillium tricorpus causing lettuce wilt in Japan differs genetically from California lettuce isolates [Elektronische Daten] [Toshiyuki Usami, Takeshi Kanto, Patrik Inderbitzin, Mizuho Itoh, Gan Kisaki, Yoshiyuki Ebihara, Wataru Suda, Yoshimiki Amemiya, Krishna Subbarao] In January 2002, Verticillium wilt of lettuce (Lactuca sativa L.) caused by Verticillium tricorpus occurred in upland paddy fields in Hyogo Prefecture for the first time in Japan. This fungal species was first isolated from lettuce in California, USA. In the present study, the genetic relationships between the American and Japanese isolates of V. tricorpus from lettuce were analyzed to determine whether the pathogen could have migrated to Japan from the USA, the major lettuce-seed supplier for Japan. Nucleotide sequences of the rDNA internal transcribed spacer regions, as well as the genes coding for translation elongation factor 1-alpha and RNA polymerase II were compared among American and Japanese V. tricorpus isolates from lettuce. The Japanese isolates of V. tricorpus were distinct from the American. Random amplified polymorphic DNA analyses also supported this conclusion. These results demonstrated that Verticillium wilt on lettuce caused by V. tricorpus in Japan was not related to the isolates causing the disease in California. The Author(s), 2010 Verticillium tricorpus nationallicence Verticillium wilt nationallicence Lettuce nationallicence Phylogenetic analysis nationallicence Random amplified polymorphic DNA (RAPD) nationallicence Usami Toshiyuki Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo, Japan aut Kanto Takeshi Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries, 679-0098, Kasai, Hyogo, Japan aut Inderbitzin Patrik Department of Plant Pathology, University of California, Davis, c/o US Agricultural Research Station, 93905, Salinas, CA, USA aut Itoh Mizuho Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo, Japan aut Kisaki Gan Kagawa Prefectural Agricultural Experiment Station, 761-8078, Takamatsu, Kagawa, Japan aut Ebihara Yoshiyuki Chiba Prefectural Agriculture and Forestry Research Center, Southern Horticulture Institute, 294-0014, Tateyama, Chiba, Japan aut Suda Wataru Graduate School of Frontier Sciences, The University of Tokyo, 277-8561, Kashiwa, Chiba, Japan aut Amemiya Yoshimiki Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo, Japan aut Subbarao Krishna Department of Plant Pathology, University of California, Davis, c/o US Agricultural Research Station, 93905, Salinas, CA, USA aut Journal of General Plant Pathology Springer Japan 77/1(2011-01-01), 17-23 1345-2630 77:1<17 2011 77 10327 https://doi.org/10.1007/s10327-010-0282-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-010-0282-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Usami Toshiyuki Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo, Japan aut NATIONALLICENCE 700 1- Kanto Takeshi Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries, 679-0098, Kasai, Hyogo, Japan aut NATIONALLICENCE 700 1- Inderbitzin Patrik Department of Plant Pathology, University of California, Davis, c/o US Agricultural Research Station, 93905, Salinas, CA, USA aut NATIONALLICENCE 700 1- Itoh Mizuho Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo, Japan aut NATIONALLICENCE 700 1- Kisaki Gan Kagawa Prefectural Agricultural Experiment Station, 761-8078, Takamatsu, Kagawa, Japan aut NATIONALLICENCE 700 1- Ebihara Yoshiyuki Chiba Prefectural Agriculture and Forestry Research Center, Southern Horticulture Institute, 294-0014, Tateyama, Chiba, Japan aut NATIONALLICENCE 700 1- Suda Wataru Graduate School of Frontier Sciences, The University of Tokyo, 277-8561, Kashiwa, Chiba, Japan aut NATIONALLICENCE 700 1- Amemiya Yoshimiki Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo, Japan aut NATIONALLICENCE 700 1- Subbarao Krishna Department of Plant Pathology, University of California, Davis, c/o US Agricultural Research Station, 93905, Salinas, CA, USA aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/1(2011-01-01), 17-23 1345-2630 77:1<17 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer