caa a22 4500 44539028X CHVBK 20180317143044.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10327-010-0275-9 doi (NATIONALLICENCE)springer-10.1007/s10327-010-0275-9 Seed and pollen transmission of Apple latent spherical virus in apple [Elektronische Daten] [Kazuya Nakamura, Noriko Yamagishi, Masamichi Isogai, Sadao Komori, Tsutae Ito, Nobuyuki Yoshikawa] To examine whether Apple latent spherical virus (ALSV) has spread among apple trees in an orchard, we surveyed 21 apple trees surrounding two ALSV-infected trees for virus infection using a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). None of the 21 trees were infected, indicating that ALSV has not spread from the infected trees to the neighboring apple trees since it was first detected in 1984. We analyzed seed embryos and seedlings derived from infected trees and detected ALSV in 10 of 223 seed embryos (4.5%) and 10 of 227 seedlings (4.4%). From these results, we conclude that ALSV is seed-transmitted at a rate of ca. 4.5% in apple. We also analyzed seed embryos and seedlings from uninfected apple trees that were hand-pollinated with pollen from infected trees. We detected ALSV in only 1 of 260 seed embryos and in none of the 227 apple seedlings. This result indicated that the seed transmission rate via infected pollen is only 0-0.38%. In situ hybridization analysis of ALSV-infected apple flower buds showed that ALSV was present inside almost all pollen grains and in all ovary and ovule tissues, including the embryo sac and inner integument. The Phytopathological Society of Japan and Springer, 2010 Apple latent spherical virus nationallicence Seed transmission nationallicence Pollen transmission nationallicence Apple nationallicence Nakamura Kazuya Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut Yamagishi Noriko Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut Isogai Masamichi Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut Komori Sadao Horticulture Laboratory, Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut Ito Tsutae Apple Research Station, National Institute of Fruit Tree Science, Shimokuriyagawa 92-24, 020-0123, Morioka, Japan aut Yoshikawa Nobuyuki Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut Journal of General Plant Pathology Springer Japan 77/1(2011-01-01), 48-53 1345-2630 77:1<48 2011 77 10327 https://doi.org/10.1007/s10327-010-0275-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-010-0275-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakamura Kazuya Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut NATIONALLICENCE 700 1- Yamagishi Noriko Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut NATIONALLICENCE 700 1- Isogai Masamichi Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut NATIONALLICENCE 700 1- Komori Sadao Horticulture Laboratory, Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut NATIONALLICENCE 700 1- Ito Tsutae Apple Research Station, National Institute of Fruit Tree Science, Shimokuriyagawa 92-24, 020-0123, Morioka, Japan aut NATIONALLICENCE 700 1- Yoshikawa Nobuyuki Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/1(2011-01-01), 48-53 1345-2630 77:1<48 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer