caa a22 4500 445390425 CHVBK 20180317143045.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10327-011-0342-x doi (NATIONALLICENCE)springer-10.1007/s10327-011-0342-x A broad spectrum, one-step RT-PCR to detect Satsuma dwarf virus variants using universal primers targeting both segmented RNAs 1 and 2 [Elektronische Daten] [Shin-ichi Shimizu, Takao Ito, Takanori Miyoshi, Yasunobu Tachibana, Tsutae Ito] Universal primers to detect Satsuma dwarf virus (SDV), including distantly related strains Citrus mosaic virus (CiMV), Navel orange infectious mottling virus (NIMV), and Hyuganatsu virus (HV), were tested in a convenient one-step RT-PCR assay. SDV was the most broadly detected using uSDVup/uSDVdo primers that specifically targeted a nucleotide sequence in the 3′-noncoding region that is conserved in both segmented RNAs 1 and 2 of SDV among the tested primers. Nucleotide sequence analysis confirmed that the amplified RT-PCR products could be derived from RNAs 1 or 2 of SDV variants, some of which had interesting genetic diversity. The Phytopathological Society of Japan and Springer, 2011 Detection nationallicence Genetic diversity nationallicence Satsuma dwarf virus nationallicence Universal primers nationallicence Shimizu Shin-ichi Fruit Tree Research Center, Ehime Research Institute of Agriculture, Forestry and Fisheries, Matsuyama, Ehime, Japan aut Ito Takao Grape and Persimmon Research Station, National Institute of Fruit Tree Science (NIFTS), 739-2494, Akitsu, Hiroshima, Japan aut Miyoshi Takanori Fruit Tree Research Center, Ehime Research Institute of Agriculture, Forestry and Fisheries, Matsuyama, Ehime, Japan aut Tachibana Yasunobu Fruit Tree Research Center, Ehime Research Institute of Agriculture, Forestry and Fisheries, Matsuyama, Ehime, Japan aut Ito Tsutae Apple Research Station, NIFTS, Iwate, Japan aut Journal of General Plant Pathology Springer Japan 77/6(2011-11-01), 326-330 1345-2630 77:6<326 2011 77 10327 https://doi.org/10.1007/s10327-011-0342-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-011-0342-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shimizu Shin-ichi Fruit Tree Research Center, Ehime Research Institute of Agriculture, Forestry and Fisheries, Matsuyama, Ehime, Japan aut NATIONALLICENCE 700 1- Ito Takao Grape and Persimmon Research Station, National Institute of Fruit Tree Science (NIFTS), 739-2494, Akitsu, Hiroshima, Japan aut NATIONALLICENCE 700 1- Miyoshi Takanori Fruit Tree Research Center, Ehime Research Institute of Agriculture, Forestry and Fisheries, Matsuyama, Ehime, Japan aut NATIONALLICENCE 700 1- Tachibana Yasunobu Fruit Tree Research Center, Ehime Research Institute of Agriculture, Forestry and Fisheries, Matsuyama, Ehime, Japan aut NATIONALLICENCE 700 1- Ito Tsutae Apple Research Station, NIFTS, Iwate, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/6(2011-11-01), 326-330 1345-2630 77:6<326 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer