caa a22 4500 445390484 CHVBK 20180317143045.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10327-011-0307-0 doi (NATIONALLICENCE)springer-10.1007/s10327-011-0307-0 Immunolocalization of Pepper mild mottle virus in developing seeds and seedlings of Capsicum annuum [Elektronische Daten] [Yoshikatsu Genda, Kyoko Sato, Osamu Nunomura, Tetsuo Hirabayashi, Shinya Tsuda] The location of Pepper mild mottle virus (PMMoV) within seeds as they developed on inoculated seedlings of pepper (Capsicum annuum) was followed over time by detecting the viral coat protein using immunofluorescence microscopy. Seedlings were inoculated with PMMoV when the flower buds on the first and second branching nodes were in bloom. Fluorescence indicating the presence of PMMoV was first observed around immature seeds and placentas in the ovaries on the fourth branching node at 20days post-anthesis (20DPA), which corresponded to 39days post-inoculation (39DPI). The area with fluorescence gradually expanded from the placenta into the integument and the parenchyma, and finally reached the tip of the immature seeds by 34DPA (53DPI). The embryo or endosperm beyond the endothelium never fluoresced during the experiment [i.e., ending at 81DPA (102DPI)]. For visualizing viral routes of invasion from seeds into new seedlings, PMMoV-infected C. annuum seeds that were heterozygous for the L 3 tobamovirus-resistance gene were sown in soil at 30°C. After ~2weeks, the cotyledon developed virally induced necrosis. These findings shed light on the infection cycle of PMMoV through vertical transmission in C. annuum. The Phytopathological Society of Japan and Springer, 2011 Immunohistochemistry nationallicence Resistance gene nationallicence Seed-borne nationallicence Seed sterilization nationallicence Soil-borne nationallicence Genda Yoshikatsu Japan Horticultural Production and Research Institute, 270-2221, Matsudo, Chiba, Japan aut Sato Kyoko Japan Horticultural Production and Research Institute, 270-2221, Matsudo, Chiba, Japan aut Nunomura Osamu Japan Horticultural Production and Research Institute, 270-2221, Matsudo, Chiba, Japan aut Hirabayashi Tetsuo Japan Horticultural Production and Research Institute, 270-2221, Matsudo, Chiba, Japan aut Tsuda Shinya Department of Plant Pathology, National Agricultural Research Center, 305-8666, Tsukuba, Ibaraki, Japan aut Journal of General Plant Pathology Springer Japan 77/3(2011-05-01), 201-208 1345-2630 77:3<201 2011 77 10327 https://doi.org/10.1007/s10327-011-0307-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-011-0307-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Genda Yoshikatsu Japan Horticultural Production and Research Institute, 270-2221, Matsudo, Chiba, Japan aut NATIONALLICENCE 700 1- Sato Kyoko Japan Horticultural Production and Research Institute, 270-2221, Matsudo, Chiba, Japan aut NATIONALLICENCE 700 1- Nunomura Osamu Japan Horticultural Production and Research Institute, 270-2221, Matsudo, Chiba, Japan aut NATIONALLICENCE 700 1- Hirabayashi Tetsuo Japan Horticultural Production and Research Institute, 270-2221, Matsudo, Chiba, Japan aut NATIONALLICENCE 700 1- Tsuda Shinya Department of Plant Pathology, National Agricultural Research Center, 305-8666, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/3(2011-05-01), 201-208 1345-2630 77:3<201 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 445390484 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer