caa a22 4500 445390492 CHVBK 20180317143045.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10327-011-0310-5 doi (NATIONALLICENCE)springer-10.1007/s10327-011-0310-5 Localization of probe-accessible chitin and characterization of genes encoding chitin-binding domains during rice- Magnaporthe oryzae interactions [Elektronische Daten] [Susumu Mochizuki, Ken-ichiro Saitoh, Eiichi Minami, Yoko Nishizawa] We examined the localization of probe-accessible chitin in Magnaporthe oryzae, which causes rice blast disease, during the early infection process and the functions of two genes encoding a chitin-binding domain (ChBD). Invasive hyphae in the first-invaded rice cell showed little staining with fluorescently labeled wheat germ agglutinin, a probe to detect chitin. However, in the second-invaded cell, hyphae showed strong fluorescence, and treatment with chitinase diminished the signal. Fourteen ChBD genes encoding family 18 carbohydrate-binding module (CBM18) were isolated from a Japanese strain of M. oryzae, Ina86-137. Reverse transcription-polymerase chain reaction analysis demonstrated that ChBD-1, ChBD-6, ChBD-8, ChBD-13, and ChBD-15 are expressed in the rice sheath. Gene-targeted disruptants of ChBD-1 and ChBD-15 had no significant differences in invasive growth, pathogenicity, or tolerance to chitinase compared to the wild type. These results suggest that M. oryzae has a mechanism to evade being a substrate for the host chitinase in the first-invaded cell, but neither ChBD-1 nor ChBD-15 contributes to this mechanism. The Author(s), 2011 CBM18 nationallicence Chitin-binding domain nationallicence Chitin elicitor nationallicence Fungal cell wall nationallicence Magnaporthe oryzae nationallicence Rice nationallicence Mochizuki Susumu Plant-Microbe Interactions Research Unit, Division of Plant Sciences, National Institute of Agrobiological Sciences, Kannondai 2-1-2, 305-8602, Tsukuba, Ibaraki, Japan aut Saitoh Ken-ichiro Plant-Microbe Interactions Research Unit, Division of Plant Sciences, National Institute of Agrobiological Sciences, Kannondai 2-1-2, 305-8602, Tsukuba, Ibaraki, Japan aut Minami Eiichi Plant-Microbe Interactions Research Unit, Division of Plant Sciences, National Institute of Agrobiological Sciences, Kannondai 2-1-2, 305-8602, Tsukuba, Ibaraki, Japan aut Nishizawa Yoko Plant-Microbe Interactions Research Unit, Division of Plant Sciences, National Institute of Agrobiological Sciences, Kannondai 2-1-2, 305-8602, Tsukuba, Ibaraki, Japan aut Journal of General Plant Pathology Springer Japan 77/3(2011-05-01), 163-173 1345-2630 77:3<163 2011 77 10327 https://doi.org/10.1007/s10327-011-0310-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-011-0310-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mochizuki Susumu Plant-Microbe Interactions Research Unit, Division of Plant Sciences, National Institute of Agrobiological Sciences, Kannondai 2-1-2, 305-8602, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Saitoh Ken-ichiro Plant-Microbe Interactions Research Unit, Division of Plant Sciences, National Institute of Agrobiological Sciences, Kannondai 2-1-2, 305-8602, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Minami Eiichi Plant-Microbe Interactions Research Unit, Division of Plant Sciences, National Institute of Agrobiological Sciences, Kannondai 2-1-2, 305-8602, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Nishizawa Yoko Plant-Microbe Interactions Research Unit, Division of Plant Sciences, National Institute of Agrobiological Sciences, Kannondai 2-1-2, 305-8602, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/3(2011-05-01), 163-173 1345-2630 77:3<163 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer