caa a22 4500 445390549 CHVBK 20180317143045.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10327-011-0304-3 doi (NATIONALLICENCE)springer-10.1007/s10327-011-0304-3 A plastidic glucose-6-phosphate dehydrogenase is responsible for hypersensitive response cell death and reactive oxygen species production [Elektronische Daten] [Shuta Asai, Miki Yoshioka, Hironari Nomura, Chiyori Tone, Kazumi Nakajima, Eiichi Nakane, Noriyuki Doke, Hirofumi Yoshioka] Glucose-6-phosphate dehydrogenase (G6PDH) has been implicated in the supply of reduced nicotine amide cofactors for resistance to biotic and abiotic stresses. Here, we show participation of the plastidic P2 isoform of G6PDH in plant immunity. A cytosolic isoform (NbG6PDH-Cyto) and two plastidic isoforms (NbG6PDH-P1 and NbG6PDH-P2) cloned from Nicotiana benthamiana were localized in cytosol and chloroplasts, respectively. Hypersensitive response (HR) cell death and NADPH oxidase (RBOH; respiratory burst oxidase homolog)-dependent reactive oxygen species (ROS) production after recognition of INF1 elicitin, secreted by oomycete Phytophthora infestans, decreased in NbG6PDH-P2-silenced plants, but not in NbG6PDH-Cyto- and NbG6PDH-P1-silenced plants. Silencing of the cytosolic NAD kinase NbNADK1, which phosphorylates NADH to form NADPH, compromised HR cell death and ROS production, and concomitant silencing with NbG6PDH-P2 reduced HR cell death and ROS to levels near those in NbG6PDH-P2-silenced plants. Similarly, silencing NbG6PDH-P2 and NbNADK1 resulted in high susceptibility to P. infestans. These results suggest that NADPH produced by the P2 isoform of G6PDH in chloroplasts is responsible for HR cell death and ROS production mediated by RBOH and that NbNADK1 is involved in this pathway. The Phytopathological Society of Japan and Springer, 2011 G6PDH nationallicence HR cell death nationallicence ROS burst nationallicence NAD kinase nationallicence NADPH oxidase nationallicence Phytophthora infestans nationallicence Asai Shuta The Sainsbury Laboratory, John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK aut Yoshioka Miki Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut Nomura Hironari Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut Tone Chiyori Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut Nakajima Kazumi Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut Nakane Eiichi Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut Doke Noriyuki Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut Yoshioka Hirofumi Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut Journal of General Plant Pathology Springer Japan 77/3(2011-05-01), 152-162 1345-2630 77:3<152 2011 77 10327 https://doi.org/10.1007/s10327-011-0304-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-011-0304-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Asai Shuta The Sainsbury Laboratory, John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK aut NATIONALLICENCE 700 1- Yoshioka Miki Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut NATIONALLICENCE 700 1- Nomura Hironari Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut NATIONALLICENCE 700 1- Tone Chiyori Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut NATIONALLICENCE 700 1- Nakajima Kazumi Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut NATIONALLICENCE 700 1- Nakane Eiichi Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut NATIONALLICENCE 700 1- Doke Noriyuki Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut NATIONALLICENCE 700 1- Yoshioka Hirofumi Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/3(2011-05-01), 152-162 1345-2630 77:3<152 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer