caa a22 4500 445390565 CHVBK 20180317143045.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10327-011-0315-0 doi (NATIONALLICENCE)springer-10.1007/s10327-011-0315-0 The presence of double-stranded RNAs in Alternaria alternata Japanese pear pathotype is associated with morphological changes [Elektronische Daten] [Kuko Fuke, Kana Takeshita, Nanako Aoki, Toshiyuki Fukuhara, Mayumi Egusa, Motoichiro Kodama, Hiromitsu Moriyama] Strains of the Japanese pear pathotype of Alternaria alternata were screened for double-stranded RNAs (dsRNAs). Four strains had several dsRNAs; strain N18 was associated with several dsRNAs and had impaired growth phenotypes such as irregular mycelium and abnormal pigmentation. We isolated dsRNA-cured isolates from strain N18 by single-conidium isolation. The dsRNA-cured isolates had recovered normal growth and pigmentation. Enlarged vesicles were observed in mycelial cells of the original dsRNA-carrying N18 strain. DAPI nuclear staining revealed regression of the nuclei in dsRNA-carrying N18 cells. These results indicate that the dsRNAs might have negative effects, such as apoptosis-like cell death, on the host fungus. The Phytopathological Society of Japan and Springer, 2011 Double-stranded RNA nationallicence Mycovirus nationallicence Alternaria alternata nationallicence Apoptosis-like cell death nationallicence Autophage nationallicence Fuke Kuko Laboratories of Molecular and Cellular Biology, Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, 183-8509, Fuchu, Tokyo, Japan aut Takeshita Kana Laboratories of Molecular and Cellular Biology, Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, 183-8509, Fuchu, Tokyo, Japan aut Aoki Nanako Laboratories of Molecular and Cellular Biology, Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, 183-8509, Fuchu, Tokyo, Japan aut Fukuhara Toshiyuki Laboratories of Molecular and Cellular Biology, Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, 183-8509, Fuchu, Tokyo, Japan aut Egusa Mayumi Laboratory of Plant Pathology, Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, 680-8553, Tottori, Japan aut Kodama Motoichiro Laboratory of Plant Pathology, Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, 680-8553, Tottori, Japan aut Moriyama Hiromitsu Laboratories of Molecular and Cellular Biology, Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, 183-8509, Fuchu, Tokyo, Japan aut Journal of General Plant Pathology Springer Japan 77/4(2011-07-01), 248-252 1345-2630 77:4<248 2011 77 10327 https://doi.org/10.1007/s10327-011-0315-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-011-0315-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fuke Kuko Laboratories of Molecular and Cellular Biology, Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, 183-8509, Fuchu, Tokyo, Japan aut NATIONALLICENCE 700 1- Takeshita Kana Laboratories of Molecular and Cellular Biology, Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, 183-8509, Fuchu, Tokyo, Japan aut NATIONALLICENCE 700 1- Aoki Nanako Laboratories of Molecular and Cellular Biology, Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, 183-8509, Fuchu, Tokyo, Japan aut NATIONALLICENCE 700 1- Fukuhara Toshiyuki Laboratories of Molecular and Cellular Biology, Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, 183-8509, Fuchu, Tokyo, Japan aut NATIONALLICENCE 700 1- Egusa Mayumi Laboratory of Plant Pathology, Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, 680-8553, Tottori, Japan aut NATIONALLICENCE 700 1- Kodama Motoichiro Laboratory of Plant Pathology, Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, 680-8553, Tottori, Japan aut NATIONALLICENCE 700 1- Moriyama Hiromitsu Laboratories of Molecular and Cellular Biology, Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, 183-8509, Fuchu, Tokyo, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/4(2011-07-01), 248-252 1345-2630 77:4<248 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer