caa a22 4500 44539059X CHVBK 20180317143045.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10327-011-0318-x doi (NATIONALLICENCE)springer-10.1007/s10327-011-0318-x Attenuated plant viruses: preventing virus diseases and understanding the molecular mechanism [Elektronische Daten] [Masamichi Nishiguchi, Kappei Kobayashi] Attenuated viruses have been isolated and studied not only as a practical means of controlling virus diseases but also to gain a molecular understanding of viral virulence and cross protection. They have been isolated from crop fields and generated through high/low temperature treatment or by mutagens such as nitrous acid and ultraviolet irradiation. Some viruses have been beneficially used in fields and evaluated for one or more decades. Molecular genetic studies on attenuated viruses have revealed that amino acid substitutions are located in replicase and the movement protein in tobamovirus, protein 2b for cucumovirus, and P1 and HC-Pro for potyvirus. In most cases, with a few exceptions, symptom attenuation is positively correlated with a reduced level of RNA silencing suppression. Molecular mechanisms underlying virus attenuation and cross protection and the rationale for practical use of attenuated viruses for effective virus disease control are discussed. The Phytopathological Society of Japan and Springer, 2011 Attenuated virus nationallicence Virulence nationallicence Cross protection nationallicence Interference nationallicence RNA silencing nationallicence Posttranscriptional gene silencing (PTGS) nationallicence Viral silencing suppressor nationallicence Nishiguchi Masamichi 246-Ehime University, 3-5-7 Tarumi, 790-8566, Matsuyama, Japan aut Kobayashi Kappei 246-Ehime University, 3-5-7 Tarumi, 790-8566, Matsuyama, Japan aut Journal of General Plant Pathology Springer Japan 77/4(2011-07-01), 221-229 1345-2630 77:4<221 2011 77 10327 https://doi.org/10.1007/s10327-011-0318-x text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-011-0318-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nishiguchi Masamichi 246-Ehime University, 3-5-7 Tarumi, 790-8566, Matsuyama, Japan aut NATIONALLICENCE 700 1- Kobayashi Kappei 246-Ehime University, 3-5-7 Tarumi, 790-8566, Matsuyama, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/4(2011-07-01), 221-229 1345-2630 77:4<221 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer