caa a22 4500 445390603 CHVBK 20180317143045.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10327-011-0311-4 doi (NATIONALLICENCE)springer-10.1007/s10327-011-0311-4 Bchex virulence gene of Botrytis cinerea [Elektronische Daten] characterization and functional analysis [C. Aguayo, J. Riquelme, P. Valenzuela, M. Hahn, E. Silva Moreno] We previously identified Bchex as a highly expressed gene during filamentous growth in Botrytis cinerea. The gene encodes the principal protein of the Woronin body and has been shown to seal septal pores in response to cellular damage. In the present study, Southern blot analysis of genomic DNA indicated that the gene exists as a single copy in the B. cinerea genome. The gene was differentially expressed during various developmental stages: expression was high in germinating conidia and the mycelial stage and lower in resting conidia and the appressorial stage. For functional analyses, homologous recombination was used to obtain a ΔBchex knockout mutant. Growth of the mutant was strongly reduced growth in complete medium and in defined media with sucrose, fructose or pectin as the carbon source. After detached tomato leaves were inoculated with the Bchex mutant, lesion development was markedly reduced compared to the control, suggesting that Bchex participates in normal growth, germination and virulence of this fungus. The Phytopathological Society of Japan and Springer, 2011 Botrytis cinerea nationallicence Woronin body nationallicence Virulence nationallicence Aguayo C. Fundación Ciencia para la Vida, Av. Zanartu 1482, Nunoa, Santiago, Chile aut Riquelme J. Fundación Ciencia para la Vida, Av. Zanartu 1482, Nunoa, Santiago, Chile aut Valenzuela P. Fundación Ciencia para la Vida, Av. Zanartu 1482, Nunoa, Santiago, Chile aut Hahn M. Department of Biology, Kaiserslautern University, PO Box 3049, 67653, Kaiserslautern, Germany aut Silva Moreno E. Fundación Ciencia para la Vida, Av. Zanartu 1482, Nunoa, Santiago, Chile aut Journal of General Plant Pathology Springer Japan 77/4(2011-07-01), 230-238 1345-2630 77:4<230 2011 77 10327 https://doi.org/10.1007/s10327-011-0311-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-011-0311-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aguayo C. Fundación Ciencia para la Vida, Av. Zanartu 1482, Nunoa, Santiago, Chile aut NATIONALLICENCE 700 1- Riquelme J. Fundación Ciencia para la Vida, Av. Zanartu 1482, Nunoa, Santiago, Chile aut NATIONALLICENCE 700 1- Valenzuela P. Fundación Ciencia para la Vida, Av. Zanartu 1482, Nunoa, Santiago, Chile aut NATIONALLICENCE 700 1- Hahn M. Department of Biology, Kaiserslautern University, PO Box 3049, 67653, Kaiserslautern, Germany aut NATIONALLICENCE 700 1- Silva Moreno E. Fundación Ciencia para la Vida, Av. Zanartu 1482, Nunoa, Santiago, Chile aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/4(2011-07-01), 230-238 1345-2630 77:4<230 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer