caa a22 4500 445390662 CHVBK 20180317143045.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10327-011-0335-9 doi (NATIONALLICENCE)springer-10.1007/s10327-011-0335-9 Quantitative nested real-time PCR detection of Verticillium longisporum and V. dahliae in the soil of cabbage fields [Elektronische Daten] [Shinpei Banno, Hidenari Saito, Hiroshi Sakai, Toshihiko Urushibara, Kentaro Ikeda, Takeshi Kabe, Isao Kemmochi, Makoto Fujimura] Verticillium longisporum and V. dahliae, causal agents of Verticillium wilt, are spreading through the cabbage fields of Gunma Prefecture. Using the V. longisporum-specific intron within the 18S rDNA and differences between ITS 5.8S rDNA sequences in Japanese isolates of V. longisporum and V. dahliae, we developed three quantitative nested real-time (QNRT) PCR assays. The QNRT-PCR quantification of V. longisporum or V. dahliae in cabbage field soil was consistent with the severity of Verticillium wilt disease in those fields. In field trials of resistant cultivar YR Ranpo grown for three seasons in soil infested with the pathogen, disease severity and pathogen density in the soil were significantly reduced in a field moderately contaminated by V. dahliae, but only slightly reduced in a highly contaminated field. These results suggest that continuous cultivation of a resistant cultivar is an effective way to reduce the pathogen population. QNRT-PCR assays provide a powerful analytical tool to evaluate the soil population dynamics of V. longisporum and V. dahliae for disease management. The Phytopathological Society of Japan and Springer, 2011 Verticillium longisporum nationallicence qPCR nationallicence Brassica oleracea L. var. capitata nationallicence Disease-resistant cultivar nationallicence Banno Shinpei Plant Regulation Research Center, Toyo University, 1-1-1 Izumino, Itakura, 374-0193, Ora, Gunma, Japan aut Saito Hidenari Plant Regulation Research Center, Toyo University, 1-1-1 Izumino, Itakura, 374-0193, Ora, Gunma, Japan aut Sakai Hiroshi Gunma Agricultural Technology Center, 493 Nishi-Obokata, 379-2224, Isesaki, Gunma, Japan aut Urushibara Toshihiko Gunma Agricultural Technology Center, 493 Nishi-Obokata, 379-2224, Isesaki, Gunma, Japan aut Ikeda Kentaro Plant Regulation Research Center, Toyo University, 1-1-1 Izumino, Itakura, 374-0193, Ora, Gunma, Japan aut Kabe Takeshi Gunma Agricultural Technology Center, 493 Nishi-Obokata, 379-2224, Isesaki, Gunma, Japan aut Kemmochi Isao Gunma Agricultural Technology Center, 493 Nishi-Obokata, 379-2224, Isesaki, Gunma, Japan aut Fujimura Makoto Plant Regulation Research Center, Toyo University, 1-1-1 Izumino, Itakura, 374-0193, Ora, Gunma, Japan aut Journal of General Plant Pathology Springer Japan 77/5(2011-09-01), 282-291 1345-2630 77:5<282 2011 77 10327 https://doi.org/10.1007/s10327-011-0335-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-011-0335-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Banno Shinpei Plant Regulation Research Center, Toyo University, 1-1-1 Izumino, Itakura, 374-0193, Ora, Gunma, Japan aut NATIONALLICENCE 700 1- Saito Hidenari Plant Regulation Research Center, Toyo University, 1-1-1 Izumino, Itakura, 374-0193, Ora, Gunma, Japan aut NATIONALLICENCE 700 1- Sakai Hiroshi Gunma Agricultural Technology Center, 493 Nishi-Obokata, 379-2224, Isesaki, Gunma, Japan aut NATIONALLICENCE 700 1- Urushibara Toshihiko Gunma Agricultural Technology Center, 493 Nishi-Obokata, 379-2224, Isesaki, Gunma, Japan aut NATIONALLICENCE 700 1- Ikeda Kentaro Plant Regulation Research Center, Toyo University, 1-1-1 Izumino, Itakura, 374-0193, Ora, Gunma, Japan aut NATIONALLICENCE 700 1- Kabe Takeshi Gunma Agricultural Technology Center, 493 Nishi-Obokata, 379-2224, Isesaki, Gunma, Japan aut NATIONALLICENCE 700 1- Kemmochi Isao Gunma Agricultural Technology Center, 493 Nishi-Obokata, 379-2224, Isesaki, Gunma, Japan aut NATIONALLICENCE 700 1- Fujimura Makoto Plant Regulation Research Center, Toyo University, 1-1-1 Izumino, Itakura, 374-0193, Ora, Gunma, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/5(2011-09-01), 282-291 1345-2630 77:5<282 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer