caa a22 4500 445390670 CHVBK 20180317143045.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10327-011-0331-0 doi (NATIONALLICENCE)springer-10.1007/s10327-011-0331-0 Inhibition of prepenetration processes of the powdery mildew Golovinomyces orontii on host inflorescence stems is reduced in the Arabidopsis cuticular mutant cer3 but not in cer1 [Elektronische Daten] [Noriko Inada, Elizabeth Savory] The obligate biotrophic fungus Golovinomyces orontii causes powdery mildew (PM) disease on its host Arabidopsis thaliana. Most research on the G. orontii-Arabidopsis pathosystem uses rosette leaves as experimental materials, so little is known about the behavior of G. orontii on other Arabidopsis organs. We thus conducted microscopic analyses of the PM infection process on leaves, stems, fruits and roots of Arabidopsis. Adaxial and abaxial surfaces of mature rosette leaves supported G. orontii infection, but growth was somewhat suppressed on cauline leaves. Prepenetration processes (germination and appressorium formation) were strongly inhibited on stems, fruits and roots. To test the effect of alterations in the Arabidopsis cuticle on the inhibition of prepenetration processes on stems, we used cuticle mutants of Arabidopsis eceriferum (cer) cer1 and cer3. Both mutants had been characterized for reduction in cuticular wax, but our chlorophyll leaching assay revealed increased cuticle permeability only in cer3. Mature rosette leaves and stems of cer1 and cer3 were inoculated, and prepenetration inhibition on stems was found to be significantly reduced in cer3 but not in cer1. We discuss the function of the cuticle in prepenetration inhibition, as well as our finding on suppression of G. orontii growth and reproduction on cer3 rosette leaves. The Phytopathological Society of Japan and Springer, 2011 Arabidopsis nationallicence Powdery mildew nationallicence Golovinomyces orontii nationallicence Cuticle nationallicence cer1 nationallicence cer3 nationallicence cer : eceriferum nationallicence dpi : Days post inoculation nationallicence ECM : Extracellular matrix nationallicence JA : Jasmonic acid nationallicence PM : Powdery mildew nationallicence SA : Salicylic acid nationallicence Inada Noriko Laboratory of Plant Function Analysis, Plant Global Educational Project, Graduate Schools of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, 630-0192, Ikoma, Nara, Japan aut Savory Elizabeth Department of Plant Pathology, 104 Center for Integrated Plant Systems, Michigan State University, 48824, East Lansing, MI, USA aut Journal of General Plant Pathology Springer Japan 77/5(2011-09-01), 273-281 1345-2630 77:5<273 2011 77 10327 https://doi.org/10.1007/s10327-011-0331-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-011-0331-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Inada Noriko Laboratory of Plant Function Analysis, Plant Global Educational Project, Graduate Schools of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, 630-0192, Ikoma, Nara, Japan aut NATIONALLICENCE 700 1- Savory Elizabeth Department of Plant Pathology, 104 Center for Integrated Plant Systems, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/5(2011-09-01), 273-281 1345-2630 77:5<273 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer