caa a22 4500 445390700 CHVBK 20180317143045.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10327-011-0322-1 doi (NATIONALLICENCE)springer-10.1007/s10327-011-0322-1 Characterization of Pectobacterium carotovorum causing a new soft rot disease on okra in Malaysia [Elektronische Daten] [Eisa Nazerian, Kamaruzaman Sijam, Zainal Meor Ahmad, Kavous Keshavarz] Dark brown, necrotic pods with extensive water-soaked lesions caused by plant pathogenic bacteria were found on okra plants in different fields in Malaysia in 2010. PCR amplification of the pectate lyase (pel) gene and amplification of the 16S-23S rRNA (ITS) with G1 and L1 primers produced 434-, 535- and 570-bp fragments, respectively. From the similarity between the results of biochemical tests and their equivalency with standard bacteriological sources, PCR-based pel gene, and RFLP analysis of the ITS-PCR products, all isolates were identified as Pectobacterium carotovorum. This is the first report of P. carotovorum in okra from Malaysia. The Phytopathological Society of Japan and Springer, 2011 Vegetables nationallicence Lady's fingers nationallicence Bacterial soft rot nationallicence Identification nationallicence ITS-RFLP nationallicence Nazerian Eisa Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia aut Sijam Kamaruzaman Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia aut Meor Ahmad Zainal Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia aut Keshavarz Kavous Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia aut Journal of General Plant Pathology Springer Japan 77/5(2011-09-01), 292-294 1345-2630 77:5<292 2011 77 10327 https://doi.org/10.1007/s10327-011-0322-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-011-0322-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nazerian Eisa Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia aut NATIONALLICENCE 700 1- Sijam Kamaruzaman Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia aut NATIONALLICENCE 700 1- Meor Ahmad Zainal Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia aut NATIONALLICENCE 700 1- Keshavarz Kavous Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/5(2011-09-01), 292-294 1345-2630 77:5<292 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer