caa a22 4500 445390719 CHVBK 20180317143045.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10327-011-0323-0 doi (NATIONALLICENCE)springer-10.1007/s10327-011-0323-0 Histochemical detection of Blueberry latent virus in highbush blueberry plant [Elektronische Daten] [Masamichi Isogai, Tatuto Nakamura, Koji Ishii, Manabu Watanabe, Noriko Yamagishi, Nobuyuki Yoshikawa] Blueberry latent virus (BBLV) was detected in 27 of 95 asymptomatic highbush blueberry trees in a blueberry field in Japan. In situ hybridization showed that the viral RNAs were detected in the palisade mesophyll, spongy mesophyll and vascular bundle of leaves. In western blot analysis, an antibody to the viral protein encoded by open reading frame 1 (ORF1) reacted with a 48-kDa protein specific for blueberry trees in which BBLV was detected. Immunogold electron microscopy revealed that amorphous bodies in the cytoplasm of blueberry cells were labeled with antibodies to the ORF1 protein. The Phytopathological Society of Japan and Springer, 2011 Blueberry latent virus nationallicence dsRNA virus nationallicence Blueberry nationallicence Isogai Masamichi Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, 020-8550, Morioka, Japan aut Nakamura Tatuto Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, 020-8550, Morioka, Japan aut Ishii Koji Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, 020-8550, Morioka, Japan aut Watanabe Manabu Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, 020-8550, Morioka, Japan aut Yamagishi Noriko Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, 020-8550, Morioka, Japan aut Yoshikawa Nobuyuki Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, 020-8550, Morioka, Japan aut Journal of General Plant Pathology Springer Japan 77/5(2011-09-01), 304-306 1345-2630 77:5<304 2011 77 10327 https://doi.org/10.1007/s10327-011-0323-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-011-0323-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Isogai Masamichi Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, 020-8550, Morioka, Japan aut NATIONALLICENCE 700 1- Nakamura Tatuto Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, 020-8550, Morioka, Japan aut NATIONALLICENCE 700 1- Ishii Koji Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, 020-8550, Morioka, Japan aut NATIONALLICENCE 700 1- Watanabe Manabu Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, 020-8550, Morioka, Japan aut NATIONALLICENCE 700 1- Yamagishi Noriko Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, 020-8550, Morioka, Japan aut NATIONALLICENCE 700 1- Yoshikawa Nobuyuki Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, 020-8550, Morioka, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/5(2011-09-01), 304-306 1345-2630 77:5<304 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer