caa a22 4500 445390735 CHVBK 20180317143046.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10327-011-0334-x doi (NATIONALLICENCE)springer-10.1007/s10327-011-0334-x Improved multiple rapid immunofilter paper assay to detect three viruses of cucumber simultaneously [Elektronische Daten] [Hideki Osaki, Koji Nomiyama, Koichi Ishikawa] We improved a multiple rapid immunofilter paper assay (multi-RIPA) to simultaneously detect three major viruses of cucumber, Cucumber mosaic virus, Melon yellow spot virus and Zucchini yellow mosaic virus. When we increased the concentration of bovine serum albumin (BSA) in the extraction buffer, a positive band for each virus developed in the multi-RIPA, regardless of the order that the three sensitized white latex particles were immobilized on the strip. The increase in BSA concentration also strengthened the sensitivity of each RIPA. In addition, using a mixture of commercially available, red and blue, sensitized latex solutions as a tracer, purple bands could be produced on the strips. Because each virus yielded an unique color for a positive reaction, the three viruses were easy to distinguish. The Phytopathological Society of Japan and Springer, 2011 Cucumber mosaic virus nationallicence Cucumber nationallicence Multiple rapid immunofilter paper assay (multi-RIPA) nationallicence Melon yellow spot virus nationallicence Zucchini yellow mosaic virus nationallicence Osaki Hideki National Agricultural Research Center for Western Region, 6-12-1 Nishifukatsu, 721-8514, Fukuyama, Hiroshima, Japan aut Nomiyama Koji Shikoku Research Center, National Agricultural Research Center for Western Region, 1-3-1 Senyuu, 765-8508, Zentsuuji, Kagawa, Japan aut Ishikawa Koichi Shikoku Research Center, National Agricultural Research Center for Western Region, 1-3-1 Senyuu, 765-8508, Zentsuuji, Kagawa, Japan aut Journal of General Plant Pathology Springer Japan 77/5(2011-09-01), 307-311 1345-2630 77:5<307 2011 77 10327 https://doi.org/10.1007/s10327-011-0334-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-011-0334-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Osaki Hideki National Agricultural Research Center for Western Region, 6-12-1 Nishifukatsu, 721-8514, Fukuyama, Hiroshima, Japan aut NATIONALLICENCE 700 1- Nomiyama Koji Shikoku Research Center, National Agricultural Research Center for Western Region, 1-3-1 Senyuu, 765-8508, Zentsuuji, Kagawa, Japan aut NATIONALLICENCE 700 1- Ishikawa Koichi Shikoku Research Center, National Agricultural Research Center for Western Region, 1-3-1 Senyuu, 765-8508, Zentsuuji, Kagawa, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/5(2011-09-01), 307-311 1345-2630 77:5<307 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer