caa a22 4500 445390751 CHVBK 20180317143046.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10327-010-0287-5 doi (NATIONALLICENCE)springer-10.1007/s10327-010-0287-5 Production of antiserum and immunodetection of Cucurbit chlorotic yellows virus, a novel whitefly-transmitted crinivirus [Elektronische Daten] [Kenji Kubota, Tomio Usugi, Shinya Tsuda] Cucurbit chlorotic yellows virus (CCYV), a whitefly-transmitted virus, is a new member of the Crinivirus genus, that causes major economic losses in cucumber, melon and watermelon crops. To develop immunodiagnostic methods for CCYV, we produced an antiserum by immunizing rabbits with bacterially expressed recombinant coat protein of CCYV and detected CCYV from CCYV-infected plants with western blotting and immunoelectron microscopy. CCYV in extracts from infected plants was also readily detected by double antibody sandwich enzyme-linked immunosorbent assay with high sensitivity and specificity. A tissue blot immunoassay indicated that CCYV localizes in the phloem tissues of the petiole and is distributed in tiny spots within the leaf lamina. The Phytopathological Society of Japan and Springer, 2010 Bemisia tabaci nationallicence CCYV nationallicence Cucumber nationallicence DAS-ELISA nationallicence Melon nationallicence CCYV : Cucurbit chlorotic yellows virus nationallicence CP : Coat protein nationallicence Kubota Kenji National Agricultural Research Center, Kannondai, 305-8666, Tsukuba, Japan aut Usugi Tomio National Agricultural Research Center, Kannondai, 305-8666, Tsukuba, Japan aut Tsuda Shinya National Agricultural Research Center, Kannondai, 305-8666, Tsukuba, Japan aut Journal of General Plant Pathology Springer Japan 77/2(2011-03-01), 116-120 1345-2630 77:2<116 2011 77 10327 https://doi.org/10.1007/s10327-010-0287-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-010-0287-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kubota Kenji National Agricultural Research Center, Kannondai, 305-8666, Tsukuba, Japan aut NATIONALLICENCE 700 1- Usugi Tomio National Agricultural Research Center, Kannondai, 305-8666, Tsukuba, Japan aut NATIONALLICENCE 700 1- Tsuda Shinya National Agricultural Research Center, Kannondai, 305-8666, Tsukuba, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/2(2011-03-01), 116-120 1345-2630 77:2<116 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer