caa a22 4500 44539076X CHVBK 20180317143046.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10327-010-0292-8 doi (NATIONALLICENCE)springer-10.1007/s10327-010-0292-8 First report of chlorotic and necrotic disease caused by Tobacco rattle virus on Capsicum annuum in Japan [Elektronische Daten] [Yasuhiro Tomitaka, Taketo Fujimoto, Michio Nakata, Ryoji Ishikawa, Tomio Usugi, Shinya Tsuda] Severe chlorosis and necrosis were observed on Capsicum annuum in Ibaraki Prefecture, Japan. A virus strain was isolated and identified as Tobacco rattle virus (TRV) by rod-shaped viral particles observed with transmission electron microscopy and by sequence analysis with a reverse transcription-polymerase chain reaction using TRV-specific primers. The disease was reproduced by back-inoculation to green pepper seedlings and their subsequent transplant into field soil containing putative nematode vectors, Trichodorus and/or Paratrichodorus. Thus, we showed that TRV is responsible for this novel disease. This report is the first of TRV infection of C. annuum in Japan. The Phytopathological Society of Japan and Springer, 2011 Capsicum annuum nationallicence Tobacco rattle virus nationallicence Nematode nationallicence Symptom nationallicence Tomitaka Yasuhiro National Agricultural Research Center, 3-1-1, Tsukuba, 305-8666, Ibaraki, Japan aut Fujimoto Taketo Faculty of Agriculture, Kyoto University, Sakyo-ku, 606-8502, Kyoto, Japan aut Nakata Michio Ibaraki Agriculture Institute, Kasama, 319-0292, Ibaraki, Japan aut Ishikawa Ryoji Ibaraki Agriculture Institute, Kasama, 319-0292, Ibaraki, Japan aut Usugi Tomio National Agricultural Research Center, 3-1-1, Tsukuba, 305-8666, Ibaraki, Japan aut Tsuda Shinya National Agricultural Research Center, 3-1-1, Tsukuba, 305-8666, Ibaraki, Japan aut Journal of General Plant Pathology Springer Japan 77/2(2011-03-01), 136-138 1345-2630 77:2<136 2011 77 10327 https://doi.org/10.1007/s10327-010-0292-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-010-0292-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tomitaka Yasuhiro National Agricultural Research Center, 3-1-1, Tsukuba, 305-8666, Ibaraki, Japan aut NATIONALLICENCE 700 1- Fujimoto Taketo Faculty of Agriculture, Kyoto University, Sakyo-ku, 606-8502, Kyoto, Japan aut NATIONALLICENCE 700 1- Nakata Michio Ibaraki Agriculture Institute, Kasama, 319-0292, Ibaraki, Japan aut NATIONALLICENCE 700 1- Ishikawa Ryoji Ibaraki Agriculture Institute, Kasama, 319-0292, Ibaraki, Japan aut NATIONALLICENCE 700 1- Usugi Tomio National Agricultural Research Center, 3-1-1, Tsukuba, 305-8666, Ibaraki, Japan aut NATIONALLICENCE 700 1- Tsuda Shinya National Agricultural Research Center, 3-1-1, Tsukuba, 305-8666, Ibaraki, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/2(2011-03-01), 136-138 1345-2630 77:2<136 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer