caa a22 4500 445390786 CHVBK 20180317143046.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10327-011-0298-x doi (NATIONALLICENCE)springer-10.1007/s10327-011-0298-x rpoD gene expression as an indicator of bacterial pathogens in host plants [Elektronische Daten] [Mari Narusaka, Tomonori Shiraishi, Masaki Iwabuchi, Yoshihiro Narusaka] Pseudomonas, Ralstonia, and Xanthomonas are important genera of plant pathogenic bacteria that affect commercially cultivated crops. We developed an assay to assess the viability and development of bacterial pathogens in Arabidopsis thaliana by detecting rpoD mRNA using a quantitative reverse transcription-polymerase chain reaction (qRT-PCR). rpoD is a representative housekeeping gene that encodes the principal RNA polymerase sigma factor. Quantification of rpoD mRNA by qRT-PCR allowed easy detection of viability and development of the pathogens in infected plant tissue. Therefore, qRT-PCR technology provides new research opportunities for investigation of host-pathogen interactions. The Phytopathological Society of Japan and Springer, 2011 Arabidopsis thaliana nationallicence rpoD gene nationallicence Pseudomonas nationallicence Quantitative reverse transcription-PCR nationallicence Ralstonia nationallicence Xanthomonas nationallicence Narusaka Mari Research Institute for Biological Sciences, Okayama, 7549-1 Yoshikawa, Kibityuo town, Kaga-gun, 716-1241, Okayama, Japan aut Shiraishi Tomonori Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University, 1-1-1 Tsushimanaka, 700-8530, Okayama, Japan aut Iwabuchi Masaki Research Institute for Biological Sciences, Okayama, 7549-1 Yoshikawa, Kibityuo town, Kaga-gun, 716-1241, Okayama, Japan aut Narusaka Yoshihiro Research Institute for Biological Sciences, Okayama, 7549-1 Yoshikawa, Kibityuo town, Kaga-gun, 716-1241, Okayama, Japan aut Journal of General Plant Pathology Springer Japan 77/2(2011-03-01), 75-80 1345-2630 77:2<75 2011 77 10327 https://doi.org/10.1007/s10327-011-0298-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-011-0298-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Narusaka Mari Research Institute for Biological Sciences, Okayama, 7549-1 Yoshikawa, Kibityuo town, Kaga-gun, 716-1241, Okayama, Japan aut NATIONALLICENCE 700 1- Shiraishi Tomonori Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University, 1-1-1 Tsushimanaka, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Iwabuchi Masaki Research Institute for Biological Sciences, Okayama, 7549-1 Yoshikawa, Kibityuo town, Kaga-gun, 716-1241, Okayama, Japan aut NATIONALLICENCE 700 1- Narusaka Yoshihiro Research Institute for Biological Sciences, Okayama, 7549-1 Yoshikawa, Kibityuo town, Kaga-gun, 716-1241, Okayama, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/2(2011-03-01), 75-80 1345-2630 77:2<75 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer