caa a22 4500 445390808 CHVBK 20180317143046.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10327-011-0296-z doi (NATIONALLICENCE)springer-10.1007/s10327-011-0296-z Suzaki Koichi Grape and Persimmon Research Station, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization, Akitsu 301-2, 739-2494, Higashihiroshima, Hiroshima, Japan aut Improved method to induce sporulation of Colletotrichum gloeosporioides , causal fungus of grape ripe rot [Elektronische Daten] [Koichi Suzaki] Colletotrichumgloeosporioides and C. acutatum are causal agents of grape ripe rot, but with available methods, sporulation of C.gloeosporioides on plate media has been unstable and inferior to that of C. acutatum. To facilitate studies on C. gloeosporioides, I developed an improved method to induce conidiation of this fungus. Isolates of C. gloeosporioides were pre-cultured in potato dextrose broth for 1week, then pulverized in whole broth. The homogenate was then spread on diluted oatmeal agar (15-20% commercial oatmeal agar medium, 1.5% agar) plates. After the plates were cultured at 25°C under continuous light for another week, the C. gloeosporioides isolates sporulated stably on the plate medium. The Phytopathological Society of Japan and Springer, 2011 Anthracnose nationallicence Colletotrichum gloeosporioides ( Glomerella cingulata ) nationallicence Grape nationallicence Ripe rot nationallicence Sporulation nationallicence Journal of General Plant Pathology Springer Japan 77/2(2011-03-01), 81-84 1345-2630 77:2<81 2011 77 10327 https://doi.org/10.1007/s10327-011-0296-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-011-0296-z text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Suzaki Koichi Grape and Persimmon Research Station, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization, Akitsu 301-2, 739-2494, Higashihiroshima, Hiroshima, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/2(2011-03-01), 81-84 1345-2630 77:2<81 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer