caa a22 4500 445390816 CHVBK 20180317143046.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10327-011-0297-y doi (NATIONALLICENCE)springer-10.1007/s10327-011-0297-y Species-specific PCRs differentiate Rosellinia necatrix from R. compacta as the prevalent cause of white root rot in Japan [Elektronische Daten] [Shuhei Takemoto, Hitoshi Nakamura, Atsuko Sasaki, Takanori Shimane] Rosellinia compacta, described recently, resembles R. necatrix and also causes white root rot. Here a species-specific PCR was developed for R. compacta, and the two R. necatrix-specific primer sets already available were validated in terms of species specificity. PCRs using the primer sets for R. necatrix amplified specific products exclusively from R. necatrix isolates. The R. compacta-specific primer set exclusively detected R. compacta, which appears to be a rare but widely distributed species. We conclude that R. necatrix is the major cause of the disease in Japan but that the involvement of R. compacta should be studied further. The Phytopathological Society of Japan and Springer, 2011 Biogeography nationallicence Identification nationallicence Soil-borne disease nationallicence Takemoto Shuhei Plant Pathology Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 2-1 Fujimoto, 305-8605, Tsukuba, Ibaraki, Japan aut Nakamura Hitoshi Plant Pathology Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 2-1 Fujimoto, 305-8605, Tsukuba, Ibaraki, Japan aut Sasaki Atsuko Plant Pathology Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 2-1 Fujimoto, 305-8605, Tsukuba, Ibaraki, Japan aut Shimane Takanori Plant Pathology Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 2-1 Fujimoto, 305-8605, Tsukuba, Ibaraki, Japan aut Journal of General Plant Pathology Springer Japan 77/2(2011-03-01), 107-111 1345-2630 77:2<107 2011 77 10327 https://doi.org/10.1007/s10327-011-0297-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-011-0297-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takemoto Shuhei Plant Pathology Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 2-1 Fujimoto, 305-8605, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Nakamura Hitoshi Plant Pathology Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 2-1 Fujimoto, 305-8605, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Sasaki Atsuko Plant Pathology Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 2-1 Fujimoto, 305-8605, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Shimane Takanori Plant Pathology Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 2-1 Fujimoto, 305-8605, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/2(2011-03-01), 107-111 1345-2630 77:2<107 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer