caa a22 4500 445390824 CHVBK 20180317143046.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10327-010-0289-3 doi (NATIONALLICENCE)springer-10.1007/s10327-010-0289-3 Microbial community profiles in intercellular fluid of rice [Elektronische Daten] [Hideki Takahashi, Hiroyuki Sekiguchi, Toyoaki Ito, Masashi Sasahara, Noriko Hatanaka, Atsushi Ohba, Shu Hase, Sugihiro Ando, Hiroshi Hasegawa, Shigehito Takenaka] Plants harbor microorganisms that are thought to stimulate plant defense systems or promote plant growth. Individual species in these intercellular microbial communities are often not sufficiently abundant to be easily described, although some endophytic microorganisms amenable to culture have been characterized. To better understand the microbial population of plants, we collected intercellular fluid (IF) from leaf blades and sheaths of rice and subsequently isolated DNA from the IF. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S and 18S rDNA fragments amplified from IF DNA by PCR indicated that these band patterns were distinguishable from those of a leaf surface-wash fluid (SF). Analysis of a set of rDNA fragments amplified from IF DNA of rice with different genotypes, paddies or growth stages for the primary survey of overall microbial community in the IF suggested that this approach is suitable for analyzing microbial diversity in the IF from various plant samples. Actually, comparative analysis of amplified rDNA fragments of rice and other five plant species indicated that the microbial diversity in IF is likely to vary substantially among plant species. We can also use sequence analysis of 16S rDNA fragments amplified from rice IF DNA to identify species including unculturable bacteria and proteobacteria and Xanthomonas and 18S rDNA fragments to identify Tilletiaria anomala, Tilletia iowensis, Ustilago maydis and unculturable eukaryotes. Thus, IF DNA analysis seems to be a good tool to further study the microbial ecology of plants. The Phytopathological Society of Japan and Springer, 2010 Endophyte nationallicence Intercellular fluid nationallicence Microbial community nationallicence Unculturable microorganisms nationallicence Rice ( Oryza sativa ) nationallicence Takahashi Hideki Department of Life Science, Graduate School of Agricultural Science, Tohoku University, 981-8555, Sendai, Japan aut Sekiguchi Hiroyuki Rhizosphere Environment Research Team, National Agricultural Research Center for Hokkaido Region, Shinsei, Memuro, 082-0081, Kasai, Hokkaido, Japan aut Ito Toyoaki Environmental Crop Science, Graduate School of Agricultural Science, Tohoku University, 989-6711, Osaki, Japan aut Sasahara Masashi Department of Crop Protection, Miyagi Prefectural Furukawa Agricultural Experiment Station, 989-6227, Osaki, Japan aut Hatanaka Noriko Department of Crop Protection, Miyagi Prefectural Furukawa Agricultural Experiment Station, 989-6227, Osaki, Japan aut Ohba Atsushi Department of Crop Protection, Miyagi Prefectural Furukawa Agricultural Experiment Station, 989-6227, Osaki, Japan aut Hase Shu Department of Life Science, Graduate School of Agricultural Science, Tohoku University, 981-8555, Sendai, Japan aut Ando Sugihiro Department of Life Science, Graduate School of Agricultural Science, Tohoku University, 981-8555, Sendai, Japan aut Hasegawa Hiroshi National Agricultural Research Center for Tohoku Region, National Agricultural Research Organization, 960-2156, Fukushima, Japan aut Takenaka Shigehito Rhizosphere Environment Research Team, National Agricultural Research Center for Hokkaido Region, Shinsei, Memuro, 082-0081, Kasai, Hokkaido, Japan aut Journal of General Plant Pathology Springer Japan 77/2(2011-03-01), 121-131 1345-2630 77:2<121 2011 77 10327 https://doi.org/10.1007/s10327-010-0289-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-010-0289-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takahashi Hideki Department of Life Science, Graduate School of Agricultural Science, Tohoku University, 981-8555, Sendai, Japan aut NATIONALLICENCE 700 1- Sekiguchi Hiroyuki Rhizosphere Environment Research Team, National Agricultural Research Center for Hokkaido Region, Shinsei, Memuro, 082-0081, Kasai, Hokkaido, Japan aut NATIONALLICENCE 700 1- Ito Toyoaki Environmental Crop Science, Graduate School of Agricultural Science, Tohoku University, 989-6711, Osaki, Japan aut NATIONALLICENCE 700 1- Sasahara Masashi Department of Crop Protection, Miyagi Prefectural Furukawa Agricultural Experiment Station, 989-6227, Osaki, Japan aut NATIONALLICENCE 700 1- Hatanaka Noriko Department of Crop Protection, Miyagi Prefectural Furukawa Agricultural Experiment Station, 989-6227, Osaki, Japan aut NATIONALLICENCE 700 1- Ohba Atsushi Department of Crop Protection, Miyagi Prefectural Furukawa Agricultural Experiment Station, 989-6227, Osaki, Japan aut NATIONALLICENCE 700 1- Hase Shu Department of Life Science, Graduate School of Agricultural Science, Tohoku University, 981-8555, Sendai, Japan aut NATIONALLICENCE 700 1- Ando Sugihiro Department of Life Science, Graduate School of Agricultural Science, Tohoku University, 981-8555, Sendai, Japan aut NATIONALLICENCE 700 1- Hasegawa Hiroshi National Agricultural Research Center for Tohoku Region, National Agricultural Research Organization, 960-2156, Fukushima, Japan aut NATIONALLICENCE 700 1- Takenaka Shigehito Rhizosphere Environment Research Team, National Agricultural Research Center for Hokkaido Region, Shinsei, Memuro, 082-0081, Kasai, Hokkaido, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/2(2011-03-01), 121-131 1345-2630 77:2<121 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer