caa a22 4500 445390867 CHVBK 20180317143046.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10327-010-0291-9 doi (NATIONALLICENCE)springer-10.1007/s10327-010-0291-9 A method to evaluate percentage of cucurbit seeds infested with Acidovorax avenae subsp. citrulli , pathogen of bacterial fruit blotch [Elektronische Daten] [Masaharu Kubota, Naoko Hagiwara, Takashi Shirakawa] We developed a method to detect Acidovorax avenae subsp. citrulli from cucurbit seeds that is more sensitive than conventional growing-out methods. Seeds were cultivated in test tubes for 2weeks, and the bacteria grew semi-selectively on the seeds and seedlings. The plants were then vigorously shaken in water to suspend bacterial cells, and the suspension was spread on a selective medium for identifying the bacterium by colony appearance. The method is suitable for research purposes to evaluate the percentage of seeds infested with the bacteria, but not to detect bacterial contamination in commercial lots with thousands of seeds. The Phytopathological Society of Japan and Springer, 2011 Acidovorax avenae subsp. citrulli nationallicence Cucurbit nationallicence Detection nationallicence Seed nationallicence Selective medium nationallicence Kubota Masaharu National Institute of Vegetable and Tea Science, 360 Kusawa, Ano, 514-2392, Tsu, Japan aut Hagiwara Naoko National Institute of Vegetable and Tea Science, 360 Kusawa, Ano, 514-2392, Tsu, Japan aut Shirakawa Takashi Tsukuba Vegetable Research Station, National Institute of Vegetable and Tea Science, 3-1-1 Kannondai, 305-8666, Tsukuba, Japan aut Journal of General Plant Pathology Springer Japan 77/2(2011-03-01), 112-115 1345-2630 77:2<112 2011 77 10327 https://doi.org/10.1007/s10327-010-0291-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-010-0291-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kubota Masaharu National Institute of Vegetable and Tea Science, 360 Kusawa, Ano, 514-2392, Tsu, Japan aut NATIONALLICENCE 700 1- Hagiwara Naoko National Institute of Vegetable and Tea Science, 360 Kusawa, Ano, 514-2392, Tsu, Japan aut NATIONALLICENCE 700 1- Shirakawa Takashi Tsukuba Vegetable Research Station, National Institute of Vegetable and Tea Science, 3-1-1 Kannondai, 305-8666, Tsukuba, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer Japan 77/2(2011-03-01), 112-115 1345-2630 77:2<112 2011 77 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer