caa a22 4500 445794607 CHVBK 20180317145120.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10620-011-1724-9 doi (NATIONALLICENCE)springer-10.1007/s10620-011-1724-9 Pathophysiological Investigation of the Gastric Surface Mucous Gel Layer of Patients with Helicobacter pylori Infection by Using Immunoassays for Trefoil Factor Family 2 and Gastric Gland Mucous Cell-Type Mucin in Gastric Juice [Elektronische Daten] [Seiko Kubota, Kazuyoshi Yamauchi, Mitsutoshi Sugano, Kenji Kawasaki, Atsushi Sugiyama, Kenji Matsuzawa, Taiji Akamatsu, Yasukazu Ohmoto, Hiroyoshi Ota] Background: The trefoil factor family (TFF) 2 protein is produced by gastric gland mucous cells (GMCs), and the secreted TFF2 shares a mucosal barrier function with GMC-type mucin. Recently, we presented an enzyme-linked immunosorbent assay (ELISA) method for measurement of GMC-type mucin in the gastric juice. Aims: We aimed to develop an ELISA for TFF2 and to assess pathophysiological changes in the gastric surface mucous gel layer (SMGL) of patients with Helicobacter pylori infection. Methods: The distribution of TFF2 and GMC-type mucin in the SMGL was immunohistochemically determined. The ELISA for TFF2 was based on a polyclonal goat antibody. Recombinant TFF2 was employed to prepare the calibrators. TFF2 and GMC-type mucin in the gastric juice in healthy individuals (n=33) and patients with gastritis (n=37), gastric ulcer (n=16), and duodenal ulcer (n=10) were assayed using ELISA. Results: TFF2 and GMC-type mucin were immunohistochemically co-localized in the gastric SMGL and GMCs. The TFF2 levels in the patients were significantly higher than those in the healthy individuals. Further, the TFF2 levels in the H. pylori-positive patients were significantly higher than those in the H. pylori-negative patients, and decreased after the eradication of the infection. GMC-type mucin levels showed a tendency similar to that of TFF2 levels. Conclusions: The upregulation of TFF2 and GMC-type mucin secretion may reflect the response of the gastric mucosa to H. pylori-induced injuries. TFF2 and GMC-type mucin secreted into the SMGL may protect the gastric mucosa against H. pylori. Springer Science+Business Media, LLC, 2011 ELISA nationallicence Gastric juice nationallicence Helicobacter pylori nationallicence Mucin nationallicence TFF2 nationallicence Kubota Seiko Department of Laboratory Medicine, Shinshu University Hospital, 3-1-1 Asahi, 390-8621, Matsumoto, Nagano, Japan aut Yamauchi Kazuyoshi Department of Medicine, Molecular Clinical Pathology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, 305-8575, Tsukuba-shi, Ibaraki, Japan aut Sugano Mitsutoshi Department of Laboratory Medicine, Shinshu University Hospital, 3-1-1 Asahi, 390-8621, Matsumoto, Nagano, Japan aut Kawasaki Kenji Department of Laboratory Medicine, Shinshu University Hospital, 3-1-1 Asahi, 390-8621, Matsumoto, Nagano, Japan aut Sugiyama Atsushi Sugiyama Clinic, 183-3 Shimadachi, 390-0852, Matsumoto, Nagano, Japan aut Matsuzawa Kenji Department of Internal Medicine, Maruko Central General Hospital, 335-5 Kamimaruko, 386-0493, Ueda, Nagano, Japan aut Akamatsu Taiji Department of Endoscopy, Suzaka Hospital, 1332 Suzaka, 382-0091, Suzaka, Nagano, Japan aut Ohmoto Yasukazu Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno, Kawauchi-cho, 771-0192, Tokushima-shi, Tokushima, Japan aut Ota Hiroyoshi Department of Health and Medical Sciences, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, 390-8621, Matsumoto, Nagano, Japan aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/12(2011-12-01), 3498-3506 0163-2116 56:12<3498 2011 56 10620 https://doi.org/10.1007/s10620-011-1724-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-011-1724-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kubota Seiko Department of Laboratory Medicine, Shinshu University Hospital, 3-1-1 Asahi, 390-8621, Matsumoto, Nagano, Japan aut NATIONALLICENCE 700 1- Yamauchi Kazuyoshi Department of Medicine, Molecular Clinical Pathology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, 305-8575, Tsukuba-shi, Ibaraki, Japan aut NATIONALLICENCE 700 1- Sugano Mitsutoshi Department of Laboratory Medicine, Shinshu University Hospital, 3-1-1 Asahi, 390-8621, Matsumoto, Nagano, Japan aut NATIONALLICENCE 700 1- Kawasaki Kenji Department of Laboratory Medicine, Shinshu University Hospital, 3-1-1 Asahi, 390-8621, Matsumoto, Nagano, Japan aut NATIONALLICENCE 700 1- Sugiyama Atsushi Sugiyama Clinic, 183-3 Shimadachi, 390-0852, Matsumoto, Nagano, Japan aut NATIONALLICENCE 700 1- Matsuzawa Kenji Department of Internal Medicine, Maruko Central General Hospital, 335-5 Kamimaruko, 386-0493, Ueda, Nagano, Japan aut NATIONALLICENCE 700 1- Akamatsu Taiji Department of Endoscopy, Suzaka Hospital, 1332 Suzaka, 382-0091, Suzaka, Nagano, Japan aut NATIONALLICENCE 700 1- Ohmoto Yasukazu Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno, Kawauchi-cho, 771-0192, Tokushima-shi, Tokushima, Japan aut NATIONALLICENCE 700 1- Ota Hiroyoshi Department of Health and Medical Sciences, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, 390-8621, Matsumoto, Nagano, Japan aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/12(2011-12-01), 3498-3506 0163-2116 56:12<3498 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer