caa a22 4500 445794674 CHVBK 20180317145121.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10620-011-1806-8 doi (NATIONALLICENCE)springer-10.1007/s10620-011-1806-8 l -Glutamine Supplementation Prevents Myenteric Neuron Loss and Has Gliatrophic Effects in the Ileum of Diabetic Rats [Elektronische Daten] [Renata Pereira, Eleandro Tronchini, Cristiano Tashima, Eder Alves, Mariana Lima, Jacqueline Zanoni] Background: Peripheral neuropathy caused chronically by diabetes mellitus is related to exacerbation of oxidative stress and a significant reduction in important endogenous antioxidants. l-Glutamine is an amino acid involved in defense mechanisms and is a substrate for the formation of glutathione, the major endogenous cellular antioxidant. Aim: This study investigated the effects of 2% l-glutamine supplementation on peripheral diabetic neuropathy and enteric glia in the ileum in rats. Methods: Male Wistar rats were divided into four groups: normoglycemics (N), normoglycemics supplemented with l-glutamine (NG), diabetics (D), and diabetics supplemented with l-glutamine (DG). After 120days, the ileums were processed for HuC/D and S100 immunohistochemistry. Quantitative and morphometric analysis was performed. Results: Diabetes significantly reduced the number of HuC/D-immunoreactive myenteric neurons per unit area and per ganglion in group D compared with normoglycemic animals (group N). l-Glutamine (2%) prevented neuronal death induced by diabetes (group DG) compared with group D. The glial density per unit area did not change with diabetes (group D) but was significantly reduced after l-glutamine supplementation (groups NG and DG). Ganglionic glial density was similar among the four groups. The neuronal area was not altered in groups D and DG. Glial size was reduced in group D; this was reversed by l-glutamine supplementation (group DG). Conclusions: We concluded that 2% l-glutamine had neuroprotective effects directly on myenteric neurons and indirectly through glial cells, which had gliatrophic effects. Springer Science+Business Media, LLC, 2011 Enteric nervous system nationallicence Diabetic neuropathy nationallicence Ileum nationallicence Glial cells nationallicence Glutamine nationallicence Pereira Renata Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, nº 5790 Bloco H-79, CEP 87020-900, Maringá, PR, Brazil aut Tronchini Eleandro Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, nº 5790 Bloco H-79, CEP 87020-900, Maringá, PR, Brazil aut Tashima Cristiano Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, nº 5790 Bloco H-79, CEP 87020-900, Maringá, PR, Brazil aut Alves Eder Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, nº 5790 Bloco H-79, CEP 87020-900, Maringá, PR, Brazil aut Lima Mariana Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, nº 5790 Bloco H-79, CEP 87020-900, Maringá, PR, Brazil aut Zanoni Jacqueline Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, nº 5790 Bloco H-79, CEP 87020-900, Maringá, PR, Brazil aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/12(2011-12-01), 3507-3516 0163-2116 56:12<3507 2011 56 10620 https://doi.org/10.1007/s10620-011-1806-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-011-1806-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pereira Renata Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, nº 5790 Bloco H-79, CEP 87020-900, Maringá, PR, Brazil aut NATIONALLICENCE 700 1- Tronchini Eleandro Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, nº 5790 Bloco H-79, CEP 87020-900, Maringá, PR, Brazil aut NATIONALLICENCE 700 1- Tashima Cristiano Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, nº 5790 Bloco H-79, CEP 87020-900, Maringá, PR, Brazil aut NATIONALLICENCE 700 1- Alves Eder Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, nº 5790 Bloco H-79, CEP 87020-900, Maringá, PR, Brazil aut NATIONALLICENCE 700 1- Lima Mariana Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, nº 5790 Bloco H-79, CEP 87020-900, Maringá, PR, Brazil aut NATIONALLICENCE 700 1- Zanoni Jacqueline Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, nº 5790 Bloco H-79, CEP 87020-900, Maringá, PR, Brazil aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/12(2011-12-01), 3507-3516 0163-2116 56:12<3507 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer