caa a22 4500 44579495X CHVBK 20180317145121.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10620-010-1346-7 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1346-7 Over-Expression of Ephb4 Is Associated with Carcinogenesis of Gastric Cancer [Elektronische Daten] [M. Li, Z. Zhao, Y. Zhang, Y. Xin] Background: Gastric cancer is one of the most frequently diagnosed malignancies in the world. The gene expression profile and molecular grouping of gastric cancer has been a challenging task due to its inherent complexity and variation among individuals. Aims: To determine the molecular mechanism associated with gastric carcinogenesis. Methods: We analyzed the gene expression profiles of 20 cancerous tissues and their tumor-adjacent tissue from patients with gastric cancer by using a 14K cDNA microarray. The differentially expressed genes and their products were verified by semiquantitative reverse transcription PCR (RT-PCR), western blotting and immunohistochemistry of gastric cancer and normal tissue samples. Results: A total of 69 genes were found to be differentially regulated in the cancerous tissue. Among them, genes such as CDH17, ETV4, S100A6, S100A11, Ephb4, and KLK10 were confirmed by RT-PCR to be up-regulated, while genes such as NK4 and PPP2R1B were down-regulated. Western blotting and immunohistochemistry indicated that Ephb4 was over-expressed and localized to the cytoplasm of gastric cancer cells. Moreover, Ephb4 protein was observed as being significantly related to tumor size and pN category (p=0.001 and 0.007, respectively). Conclusions: These newly identified genes might provide a valuable resource for understanding the molecular mechanism associated with the carcinogenesis of gastric cancer and for finding potential diagnostic markers of gastric cancer. Springer Science+Business Media, LLC, 2010 Stomach neoplasms nationallicence Biological markers nationallicence Lymph node nationallicence Ephb4 nationallicence Gastric cancer nationallicence Differential gene expression nationallicence Li M. Department of Medical Oncology, The Second Affiliated Hospital, Dalian Medical University, Dalian, Liaoning Province, China aut Zhao Z. Department of General Surgery, The First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning Province, China aut Zhang Y. Department of Medical Oncology, The Second Affiliated Hospital, Dalian Medical University, Dalian, Liaoning Province, China aut Xin Y. Cancer Institute, The First Affiliated Hospital, China Medical University, 155 Nanjing Beijie, 110001, Shenyang, Liaoning Province, China aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/3(2011-03-01), 698-706 0163-2116 56:3<698 2011 56 10620 https://doi.org/10.1007/s10620-010-1346-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1346-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li M. Department of Medical Oncology, The Second Affiliated Hospital, Dalian Medical University, Dalian, Liaoning Province, China aut NATIONALLICENCE 700 1- Zhao Z. Department of General Surgery, The First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning Province, China aut NATIONALLICENCE 700 1- Zhang Y. Department of Medical Oncology, The Second Affiliated Hospital, Dalian Medical University, Dalian, Liaoning Province, China aut NATIONALLICENCE 700 1- Xin Y. Cancer Institute, The First Affiliated Hospital, China Medical University, 155 Nanjing Beijie, 110001, Shenyang, Liaoning Province, China aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/3(2011-03-01), 698-706 0163-2116 56:3<698 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer