caa a22 4500 445795050 CHVBK 20180317145122.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10620-010-1347-6 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1347-6 Analysis of Biliary Epithelial-Mesenchymal Transition in Portal Tract Fibrogenesis in Biliary Atresia [Elektronische Daten] [Yu-Hua Deng, Cong-Lun Pu, Ying-Cun Li, Jin Zhu, Chunping Xiang, Ming-Man Zhang, Chun-Bao Guo] Background: The cellular origin of myofibroblast in the liver fibrosis remains unclear. This study was designed to investigate whether biliary epithelial cells (BECs) undergoing epithelial-mesenchymal transition (EMT) might be found in patients with biliary atresia, thereby serving as a source of fibrotic myofibroblasts. Methods: Liver sections from patients with biliary atresia were evaluated to detect antigen for the BECs marker 4 and cytokeratin-7 (CK-7), proteins (fibroblast-specific protein 1, also known S100A4; the collagen chaperone heat shock protein 47, HSP47) characteristically expressed by cells undergoing EMT, as well as myofibroblasts marker a-smooth muscle actin (a-SMA). Results: Normal bile ducts BECs could express CK-7 and low levels of a-SMA; they did not express S100A4 and HSP47. However, BECs from biliary atresia resulted in increased expression of a-SMA, S100A4, with concurrent transition to a fibroblast-like morphology and decreased expression of AK-7. Furthermore, BECs in biliary atresia were associated with significant bile ductular proliferation and coexpressed both epithelial and mesenchymal markers. Conclusions: From significant histologic evidence, the BECs forming small- and medium-sized bile ducts undergoing EMT may account for prominent bile ductular proliferation and directly contribute to fibrogenesis in BA. Springer Science+Business Media, LLC, 2010 Epithelial-mesenchymal transition nationallicence Biliary atresia nationallicence Fibrogenesis nationallicence S100A4 nationallicence EMT : Epithelial-mesenchymal transition nationallicence aSMA : a-Smooth muscle actin nationallicence BECs : Biliary epithelial cells nationallicence CK-7 : Cytokeratin-7 nationallicence Deng Yu-Hua Department of Hepatobiliary Surgery, Children's Hospital of Chongqing Medical University, 136 Zhongshan 2nd Rd., 400014, Chongqing, People's Republic of China aut Pu Cong-Lun Department of Hepatobiliary Surgery, Children's Hospital of Chongqing Medical University, 136 Zhongshan 2nd Rd., 400014, Chongqing, People's Republic of China aut Li Ying-Cun Department of Hepatobiliary Surgery, Children's Hospital of Chongqing Medical University, 136 Zhongshan 2nd Rd., 400014, Chongqing, People's Republic of China aut Zhu Jin Department of Pathology, Children's Hospital, Chongqing Medical University, 400014, Chongqing, People's Republic of China aut Xiang Chunping Department of Pathology, Children's Hospital, Chongqing Medical University, 400014, Chongqing, People's Republic of China aut Zhang Ming-Man Department of Hepatobiliary Surgery, Children's Hospital of Chongqing Medical University, 136 Zhongshan 2nd Rd., 400014, Chongqing, People's Republic of China aut Guo Chun-Bao Department of Hepatobiliary Surgery, Children's Hospital of Chongqing Medical University, 136 Zhongshan 2nd Rd., 400014, Chongqing, People's Republic of China aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/3(2011-03-01), 731-740 0163-2116 56:3<731 2011 56 10620 https://doi.org/10.1007/s10620-010-1347-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1347-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Deng Yu-Hua Department of Hepatobiliary Surgery, Children's Hospital of Chongqing Medical University, 136 Zhongshan 2nd Rd., 400014, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Pu Cong-Lun Department of Hepatobiliary Surgery, Children's Hospital of Chongqing Medical University, 136 Zhongshan 2nd Rd., 400014, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Li Ying-Cun Department of Hepatobiliary Surgery, Children's Hospital of Chongqing Medical University, 136 Zhongshan 2nd Rd., 400014, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Jin Department of Pathology, Children's Hospital, Chongqing Medical University, 400014, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Xiang Chunping Department of Pathology, Children's Hospital, Chongqing Medical University, 400014, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Ming-Man Department of Hepatobiliary Surgery, Children's Hospital of Chongqing Medical University, 136 Zhongshan 2nd Rd., 400014, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Guo Chun-Bao Department of Hepatobiliary Surgery, Children's Hospital of Chongqing Medical University, 136 Zhongshan 2nd Rd., 400014, Chongqing, People's Republic of China aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/3(2011-03-01), 731-740 0163-2116 56:3<731 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer