caa a22 4500 445795085 CHVBK 20180317145122.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10620-010-1363-6 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1363-6 Immune Phenotype of Children with Newly Diagnosed and Gluten-Free Diet-Treated Celiac Disease [Elektronische Daten] [Áron Cseh, Barna Vásárhelyi, Balázs Szalay, Kriszta Molnár, Dorottya Nagy-Szakál, András Treszl, Ádám Vannay, András Arató, Tivadar Tulassay, Gábor Veres] Background: Recent data suggest the involvement of both the adaptive and the innate immune system in celiac disease (CD). However, little is known about the immune phenotype of children with CD and its alteration upon dietary intervention. Aims: We characterized the prevalence of major interacting members of the adaptive and innate immune system in peripheral blood of newly diagnosed children with CD and tested its alteration with the improvement of clinical signs after the introduction of gluten-free diet (GFD). Methods: Peripheral blood was taken from ten children with biopsy-proven CD at the time of diagnosis and after the resolution of clinical symptoms following GFD. As controls, 15 children with functional abdominal pain were enrolled. The prevalence of the cells of adaptive and innate immunity was measured with labeled antibodies against surface markers and intracellular FoxP3 using a flow cytometer. Results: Patients with CD were found to have lower T helper, Th1 and natural killer (NK), NKT and invariant NKT cell prevalence and with higher prevalence of activated CD4+ cells, myeloid dendritic cells (DC) and Toll-like receptor (TLR) 2 and TLR-4 positive DCs and monocytes compared to controls. After resolution of symptoms on GFD, the majority of these changes normalized, although the prevalence of NK and NKT cell, DC and TLR-2 expressing DCs and monocytes remained abnormal. Conclusions: The immune phenotype in childhood CD indicates the implication of both adaptive and innate immune system. The normalization of immune abnormalities occurs on GFD, but the kinetics of this process probably differs among different cell types. Springer Science+Business Media, LLC, 2010 Celiac disease nationallicence Lymphocyte nationallicence Regulatory T cell nationallicence Dendritic cell nationallicence Monocyte nationallicence Toll-like receptor nationallicence Cseh Áron Research Group for Pediatrics and Nephrology, Semmelweis University and Hungarian Academy of Sciences, First Department of Pediatrics, Bókay u. 53, 1083, Budapest, Hungary aut Vásárhelyi Barna Research Group for Pediatrics and Nephrology, Semmelweis University and Hungarian Academy of Sciences, First Department of Pediatrics, Bókay u. 53, 1083, Budapest, Hungary aut Szalay Balázs Research Group for Pediatrics and Nephrology, Semmelweis University and Hungarian Academy of Sciences, First Department of Pediatrics, Bókay u. 53, 1083, Budapest, Hungary aut Molnár Kriszta Research Group for Pediatrics and Nephrology, Semmelweis University and Hungarian Academy of Sciences, First Department of Pediatrics, Bókay u. 53, 1083, Budapest, Hungary aut Nagy-Szakál Dorottya Research Group for Pediatrics and Nephrology, Semmelweis University and Hungarian Academy of Sciences, First Department of Pediatrics, Bókay u. 53, 1083, Budapest, Hungary aut Treszl András Research Group for Pediatrics and Nephrology, Semmelweis University and Hungarian Academy of Sciences, First Department of Pediatrics, Bókay u. 53, 1083, Budapest, Hungary aut Vannay Ádám Research Group for Pediatrics and Nephrology, Semmelweis University and Hungarian Academy of Sciences, First Department of Pediatrics, Bókay u. 53, 1083, Budapest, Hungary aut Arató András First Department of Pediatrics, Semmelweis University, Budapest, Hungary aut Tulassay Tivadar First Department of Pediatrics, Semmelweis University, Budapest, Hungary aut Veres Gábor First Department of Pediatrics, Semmelweis University, Budapest, Hungary aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/3(2011-03-01), 792-798 0163-2116 56:3<792 2011 56 10620 https://doi.org/10.1007/s10620-010-1363-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1363-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cseh Áron Research Group for Pediatrics and Nephrology, Semmelweis University and Hungarian Academy of Sciences, First Department of Pediatrics, Bókay u. 53, 1083, Budapest, Hungary aut NATIONALLICENCE 700 1- Vásárhelyi Barna Research Group for Pediatrics and Nephrology, Semmelweis University and Hungarian Academy of Sciences, First Department of Pediatrics, Bókay u. 53, 1083, Budapest, Hungary aut NATIONALLICENCE 700 1- Szalay Balázs Research Group for Pediatrics and Nephrology, Semmelweis University and Hungarian Academy of Sciences, First Department of Pediatrics, Bókay u. 53, 1083, Budapest, Hungary aut NATIONALLICENCE 700 1- Molnár Kriszta Research Group for Pediatrics and Nephrology, Semmelweis University and Hungarian Academy of Sciences, First Department of Pediatrics, Bókay u. 53, 1083, Budapest, Hungary aut NATIONALLICENCE 700 1- Nagy-Szakál Dorottya Research Group for Pediatrics and Nephrology, Semmelweis University and Hungarian Academy of Sciences, First Department of Pediatrics, Bókay u. 53, 1083, Budapest, Hungary aut NATIONALLICENCE 700 1- Treszl András Research Group for Pediatrics and Nephrology, Semmelweis University and Hungarian Academy of Sciences, First Department of Pediatrics, Bókay u. 53, 1083, Budapest, Hungary aut NATIONALLICENCE 700 1- Vannay Ádám Research Group for Pediatrics and Nephrology, Semmelweis University and Hungarian Academy of Sciences, First Department of Pediatrics, Bókay u. 53, 1083, Budapest, Hungary aut NATIONALLICENCE 700 1- Arató András First Department of Pediatrics, Semmelweis University, Budapest, Hungary aut NATIONALLICENCE 700 1- Tulassay Tivadar First Department of Pediatrics, Semmelweis University, Budapest, Hungary aut NATIONALLICENCE 700 1- Veres Gábor First Department of Pediatrics, Semmelweis University, Budapest, Hungary aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/3(2011-03-01), 792-798 0163-2116 56:3<792 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer