caa a22 4500 445795158 CHVBK 20180317145122.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10620-010-1362-7 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1362-7 The Effect of Exopolysaccharide-Producing Probiotic Strains on Gut Oxidative Damage in Experimental Colitis [Elektronische Daten] [Neriman Şengül, Sevil Işık, Belma Aslım, Gülberk Uçar, Ali Demirbağ] Background: Oxidative stress plays a role in disease initiation and progression in inflammatory bowel disease (IBD) and manipulation of this pathway may attenuate disease progress. In this study, the effect of exopolysaccharide (EPS)-producing probiotic bacteria on gut oxidative damage was evaluated in a rat model of experimental colitis. Methods: Colitis was induced by intracolonic administration of acetic acid. Rats were treated daily with two probiotic strains, L. delbrueckii subsp. bulgaricus B3 strain (EPS of 211mg/l; high-EPS group) or L. delbrueckii subsp. bulgaricus A13 strain (EPS of 27mg/l; low-EPS group), which were given directly into the stomach. Noncolitis-fed control and preventative groups were only treated with the high-EPS producing strain. Antioxidant enzyme activities (superoxide dismutase, catalase, total glutathione, reduced glutathione, glutathione disulfide) and lipid peroxidation were measured in colonic tissue samples after a treatment period of 7days. Results: Significant oxidative damage was associated with a higher level of malondialdehyde (MDA) activity and reduced antioxidant enzyme activities in the colitis model group. All antioxidant enzyme activities were higher in both probiotic-treated groups compared with those of the colitis model group (P<0.001). Lipid peroxidation was significantly ameliorated in both probiotic groups. The improvement of oxidative stress parameters was significantly more in the high-EPS group than in the low-EPS group (P<0.001). Conclusions: EPS-producing probiotic bacteria significantly attenuate oxidative stress in experimental colitis. Increased EPS production gives rise to a better probiotic function. These results suggest that EPS molecules could revaluate probiotic strains and exert their beneficial effects on the host and this may have a therapeutic potential. Springer Science+Business Media, LLC, 2010 Inflammatory bowel disease nationallicence Exopolysaccharides nationallicence Probiotic nationallicence Oxidative damage nationallicence Şengül Neriman Department of General Surgery, Faculty of Medicine, Abant İzzet Baysal University, Bolu, Emek Mah. 8. Cad 75.Sok No: 53/2, 06510, Emek, Ankara, Turkey aut Işık Sevil Department of General Surgery, Faculty of Medicine, Ordu University, Ordu, Turkey aut Aslım Belma Department of Biology, Faculty of Science and Arts, Gazi University, Ankara, Turkey aut Uçar Gülberk Department of Biochemistry, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey aut Demirbağ Ali Department of Gastrointestinal Surgery, Turkey Yuksek Ihtisas Hospital, Ankara, Turkey aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/3(2011-03-01), 707-714 0163-2116 56:3<707 2011 56 10620 https://doi.org/10.1007/s10620-010-1362-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1362-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Şengül Neriman Department of General Surgery, Faculty of Medicine, Abant İzzet Baysal University, Bolu, Emek Mah. 8. Cad 75.Sok No: 53/2, 06510, Emek, Ankara, Turkey aut NATIONALLICENCE 700 1- Işık Sevil Department of General Surgery, Faculty of Medicine, Ordu University, Ordu, Turkey aut NATIONALLICENCE 700 1- Aslım Belma Department of Biology, Faculty of Science and Arts, Gazi University, Ankara, Turkey aut NATIONALLICENCE 700 1- Uçar Gülberk Department of Biochemistry, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey aut NATIONALLICENCE 700 1- Demirbağ Ali Department of Gastrointestinal Surgery, Turkey Yuksek Ihtisas Hospital, Ankara, Turkey aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/3(2011-03-01), 707-714 0163-2116 56:3<707 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer