caa a22 4500 445795239 CHVBK 20180317145122.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10620-010-1348-5 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1348-5 MCM-2 and MCM-5 Expression in Gastric Adenocarcinoma: Clinical Significance and Comparison with Ki-67 Proliferative Marker [Elektronische Daten] [Constantinos Giaginis, Athina Giagini, Gerasimos Tsourouflis, Elisavet Gatzidou, Emmanuel Agapitos, Gregorios Kouraklis, Stamatios Theocharis] Background: Minichromosome maintenance (MCM) proteins are essential components of DNA replication, being related to cell proliferation, and serve as useful biomarkers for cancer screening, surveillance and prognosis. The aim of the present study was to evaluate the clinical significance of MCM-2 and MCM-5 expression in gastric adenocarcinoma in comparison with Ki-67 proliferative marker. Methods: MCM-2, MCM-5 and Ki-67 expression was assessed immunohistochemically in 66 tumoral samples of gastric adenocarcinoma patients and was statistically analyzed in relation to clinicopathological characteristics and patient survival. Results: MCM-2 expression did not show significant associations with any clinicopathological parameters, while Ki-67 expression was merely significantly associated with tumor size (P=0.0150). MCM-2 and Ki-67 expression were more frequently in intestinal (median values: 67.5 and 60%) compared to diffuse-type (median values: 60 and 45%) gastric adenocarcinoma cases without though reaching statistical significance (P>0.05). MCM-5 expression was significantly associated with tumor size (P=0.0295), presence of lymph node metastases (P=0.0216) and tumor histopathological stage (P=0.0098). Patients presenting high MCM-5 expression had significantly shorter survival times (log-rank test, P=0.0042), whereas neither MCM-2 nor Ki-67 expression showed significant prognostic value (log-rank test, P=0.9618 and P=0.7174, respectively). In multivariate analysis, patient age, histopathological stage and grade of differentiation, but not MCM-5 expression, were identified as independent prognostic factors (Cox regression analysis, P=0.0097, P=0.0195, P=0.0035 and P=0.3245, respectively). Conclusions: The present study showed that MCM-5 expression was associated with clinicopathological parameters in gastric adenocarcinoma. However, further studies highlighting the distinct impact of the two histopathological types, intestinal and diffuse, are warranted to delineate whether MCMs could be used as diagnostic and prognostic markers in gastric neoplasia. Springer Science+Business Media, LLC, 2010 MCM-2, MCM-5, Ki-67 nationallicence Proliferative markers nationallicence Clinicopathological parameters nationallicence Prognosis nationallicence Giaginis Constantinos Department of Forensic Medicine and Toxicology, Medical School, University of Athens, 75 Mikras Asias Street, 11527, Athens, Greece aut Giagini Athina Department of Forensic Medicine and Toxicology, Medical School, University of Athens, 75 Mikras Asias Street, 11527, Athens, Greece aut Tsourouflis Gerasimos Department of Forensic Medicine and Toxicology, Medical School, University of Athens, 75 Mikras Asias Street, 11527, Athens, Greece aut Gatzidou Elisavet Department of Forensic Medicine and Toxicology, Medical School, University of Athens, 75 Mikras Asias Street, 11527, Athens, Greece aut Agapitos Emmanuel First Department of Pathology, Medical School, University of Athens, 75, Agiou Thoma Street, 11527, Athens, Greece aut Kouraklis Gregorios Second Department of Propedeutic Surgery, Laikon Hospital, Medical School, University of Athens, 75, Agiou Thoma Street, 11527, Athens, Greece aut Theocharis Stamatios Department of Forensic Medicine and Toxicology, Medical School, University of Athens, 75 Mikras Asias Street, 11527, Athens, Greece aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/3(2011-03-01), 777-785 0163-2116 56:3<777 2011 56 10620 https://doi.org/10.1007/s10620-010-1348-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1348-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Giaginis Constantinos Department of Forensic Medicine and Toxicology, Medical School, University of Athens, 75 Mikras Asias Street, 11527, Athens, Greece aut NATIONALLICENCE 700 1- Giagini Athina Department of Forensic Medicine and Toxicology, Medical School, University of Athens, 75 Mikras Asias Street, 11527, Athens, Greece aut NATIONALLICENCE 700 1- Tsourouflis Gerasimos Department of Forensic Medicine and Toxicology, Medical School, University of Athens, 75 Mikras Asias Street, 11527, Athens, Greece aut NATIONALLICENCE 700 1- Gatzidou Elisavet Department of Forensic Medicine and Toxicology, Medical School, University of Athens, 75 Mikras Asias Street, 11527, Athens, Greece aut NATIONALLICENCE 700 1- Agapitos Emmanuel First Department of Pathology, Medical School, University of Athens, 75, Agiou Thoma Street, 11527, Athens, Greece aut NATIONALLICENCE 700 1- Kouraklis Gregorios Second Department of Propedeutic Surgery, Laikon Hospital, Medical School, University of Athens, 75, Agiou Thoma Street, 11527, Athens, Greece aut NATIONALLICENCE 700 1- Theocharis Stamatios Department of Forensic Medicine and Toxicology, Medical School, University of Athens, 75 Mikras Asias Street, 11527, Athens, Greece aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/3(2011-03-01), 777-785 0163-2116 56:3<777 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer